Journal article
Immunogenicity and In Vivo Efficacy of Recombinant Plasmodium falciparum Merozoite Surface Protein-1 in Aotus Monkeys
Molecular medicine (Cambridge, Mass.), Vol.1(3), pp.325-332
03/1995
DOI: 10.1007/BF03401557
PMCID: PMC2229908
PMID: 8529111
Abstract
Background
The carboxy-terminus of the merozoite surface protein-1 (MSP1) of Plasmodium falciparum has been implicated as a target of protective immunity.
Materials and Methods
Two recombinant proteins from the carboxy-tenriinus of MSP1, the 42 kD fused to GST (bMSP142) and the 19 kD (yMSP119), were expressed in Escherichia coli and secreted from Saccharomyces cerevisiae, respectively. To determine if vaccination with these recombinant proteins induces protective immunity, we conducted a randomized, blinded vaccine trial in two species of Aotus monkeys, A. nancymai and A. vociferans. After three injections using Freund’s adjuvant, the monkeys were challenged with the virulent Vietnam Oak Knoll (FVO) strain of P. falciparum.
Results
All three control monkeys required treatment by Day 19. Two of three monkeys vaccinated with bMSP142 required treatment by Day 17, whereas the third monkey controlled parasitemia for 28 days before requiring treatment. In contrast, both of the A. nancymai vaccinated with yMSP119 self-resolved an otherwise lethal infection. One of the two yMSP119-vaccinated A. vociferans had a prolonged prepatent period of >28 days before requiring treatment. No evidence of mutations were evident in the parasites recovered after the prolonged prepatent period. Sera from the two A. nancymai that self-cured had no detectable effect on in vitro invasion.
Conclusions
Vaccination of A. nancymai with yMSP119 induced protective immune responses. The course of recrudescing parasitemias in protected monkeys suggested that immunity is not mediated by antibodies that block invasion. Our data indicate that vaccine trials with the highly adapted FVO strain of P. falciparum can be tested in A. nancymai and that MSP119 is a promising anti-blood-stage vaccine for human trials.
Details
- Title: Subtitle
- Immunogenicity and In Vivo Efficacy of Recombinant Plasmodium falciparum Merozoite Surface Protein-1 in Aotus Monkeys
- Creators
- Sanjai KumarAnjali YadavaDavid B KeisterJing Hui TianMichael OhlKathy A Perdue-GreenfieldLouis H MillerDavid C Kaslow
- Resource Type
- Journal article
- Publication Details
- Molecular medicine (Cambridge, Mass.), Vol.1(3), pp.325-332
- DOI
- 10.1007/BF03401557
- PMID
- 8529111
- PMCID
- PMC2229908
- ISSN
- 1076-1551
- eISSN
- 1528-3658
- Language
- English
- Date published
- 03/1995
- Academic Unit
- Psychiatry; Internal Medicine
- Record Identifier
- 9984094496402771
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