Journal article
Immunoglobulin G of patients with circumscribed pretibial myxedema of Graves' disease stimulates proteoglycan synthesis in human skin fibroblasts in culture
European journal of endocrinology, Vol.127(1), pp.44-51
07/1992
DOI: 10.1530/acta.0.1270044
PMID: 1519422
Abstract
Hyaluronic acid and proteoglycan accumulate in the affected skin of Graves' disease patients with pretibial myxedema (PTM). We aimed to determine whether an autoantibody IgG circulating in PTM patients stimulates proteoglycan synthesis in human skin fibroblasts, resulting in PTM. IgGs were purified from 14 normal subjects, 11 Graves' disease patients with PTM, 5 Graves' disease patients with active ophthalmopathy and 15 Graves' disease patients with neither PTM nor ophthalmopathy. Human skin fibroblasts were incubated with the IgGs and labeled with [ 35 S]sulfate. The medium and cell layer were separated and the proteoglycan was extracted. The 35 S radioactivity in the proteoglycan fraction was measured. Compared with normal IgGs or with those of Graves' disease without PTM or ophthalmopathy, PTM IgGs significantly increased the incorporation of the 35 S into the proteoglycan. The effect of PTM IgG was dose-dependent. As PTM IgG did not alter degradation of the 35 S labeled proteoglycan, an increase in 35 S incorporation reflects increased synthesis. The effect was mediated through a mechanism other than adenylate cyclase activation. The present study demonstrates the presence of an autoantibody in PTM IgG that stimulates proteoglycan production through human skin fibroblasts. This is not correlated with the thyroid stimulating antibody activity. It is suggested that the activity of this antibody leads to the development of PTM.
Details
- Title: Subtitle
- Immunoglobulin G of patients with circumscribed pretibial myxedema of Graves' disease stimulates proteoglycan synthesis in human skin fibroblasts in culture
- Creators
- Yoshimasa Shishiba - Toranomon HospitalYumi ImaiRitsuko OdajimaYasunori OzawaTaeko Shimizu
- Resource Type
- Journal article
- Publication Details
- European journal of endocrinology, Vol.127(1), pp.44-51
- DOI
- 10.1530/acta.0.1270044
- PMID
- 1519422
- ISSN
- 0804-4643
- eISSN
- 1479-683X
- Language
- English
- Date published
- 07/1992
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984360057102771
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