Journal article
Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans
Toxicologic pathology, Vol.44(3), pp.434-441
04/2016
DOI: 10.1177/0192623315609691
PMCID: PMC4805467
PMID: 26511846
Abstract
Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues. We compare the utility of these markers between pig and human lungs to validate translational relevance of each marker. Our results suggest these markers can be a useful addition in the pathological evaluation of porcine models of human disease.
Details
- Title: Subtitle
- Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans
- Creators
- David K Meyerholz - Department of Pathology, University of Iowa, Iowa City, IA, USA david-meyerholz@uiowa.eduAllyn M Lambertz - Department of Pathology, University of Iowa, Iowa City, IA, USALeah R Reznikov - Department of Internal Medicine, University of Iowa, Iowa City, IA, USAGeorgina K Ofori-Amanfo - Department of Pathology, University of Iowa, Iowa City, IA, USAPhil H Karp - Department of Internal Medicine, University of Iowa, Iowa City, IA, USAPaul B McCray Jr - Department of Pediatrics, University of Iowa, Iowa City, IA, USAMichael J Welsh - Department of Internal Medicine, University of Iowa, Iowa City, IA, USA Department of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA, USA Howard Hughes Medical Institute, University of Iowa, Iowa City, IA, USADavid A Stoltz - Department of Internal Medicine, University of Iowa, Iowa City, IA, USA Department of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA, USA Department of Biomedical Engineering, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Toxicologic pathology, Vol.44(3), pp.434-441
- Publisher
- United States
- DOI
- 10.1177/0192623315609691
- PMID
- 26511846
- PMCID
- PMC4805467
- ISSN
- 0192-6233
- eISSN
- 1533-1601
- Grant note
- P01HL051670 / NHLBI NIH HHS P30 ES005605 / NIEHS NIH HHS P01 HL091842 / NHLBI NIH HHS DP2HL117744 / NCCDPHP CDC HHS Howard Hughes Medical Institute 1K99HL119560 / NHLBI NIH HHS P01 HL051670 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS DP2 HL117744 / NHLBI NIH HHS K99 HL119560 / NHLBI NIH HHS
- Language
- English
- Date published
- 04/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Pathology; Neurosurgery; Internal Medicine
- Record Identifier
- 9984013117702771
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