Journal article
Immunohistochemical Detection of Myeloperoxidase and Its Oxidation Products in Kupffer Cells of Human Liver
The American journal of pathology, Vol.159(6), pp.2081-2088
12/2001
DOI: 10.1016/S0002-9440(10)63059-3
PMCID: PMC1850615
PMID: 11733358
Abstract
Oxidative damage to tissue proteins has been implicated in the pathogenesis of liver disease, but the mechanisms that promote oxidation
in vivo
are unclear. Hydrogen peroxide is transformed into an array of potentially damaging reactants by the heme protein myeloperoxidase. This proinflammatory enzyme is expressed by circulating neutrophils and monocytes but is generally thought to be absent from tissue macrophages. To determine whether myeloperoxidase is present in Kupffer cells, the fixed-tissue macrophages of liver, Western blot analysis, and immunohistochemistry were performed. Two different antibodies monospecific for myeloperoxidase identified a 60-kd protein, the predicted molecular mass of myeloperoxidase, in human liver extracts. Immunostaining detected the enzyme in sinusoidal lining cells of normal and diseased human livers. Immunofluorescence confocal microscopy demonstrated co-localization of myeloperoxidase and CD68, a monocyte/macrophage marker, in sinusoidal lining cells. Numerous myeloperoxidase-expressing cells were also evident in the fibrous septa of cirrhotic livers. Immunostaining with an antibody to proteins modified by hypochlorous acid, a characteristic product of the enzyme, indicated that myeloperoxidase is enzymatically active in cases of acute liver injury and cirrhosis. These findings identify myeloperoxidase as a component of human Kupffer cells. Oxidative damage resulting from the action of myeloperoxidase may contribute to acute liver injury and hepatic fibrogenesis.
Details
- Title: Subtitle
- Immunohistochemical Detection of Myeloperoxidase and Its Oxidation Products in Kupffer Cells of Human Liver
- Creators
- Kyle E Brown - St. Louis; the Departments of Internal MedicineElizabeth M Brunt - Saint Louis University School of Medicine, St. Louis; and the Department of MedicineJay W Heinecke - Washington University School of Medicine, St. Louis, Missouri
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.159(6), pp.2081-2088
- Publisher
- American Society for Investigative Pathology
- DOI
- 10.1016/S0002-9440(10)63059-3
- PMID
- 11733358
- PMCID
- PMC1850615
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Language
- English
- Date published
- 12/2001
- Academic Unit
- Gastroenterology and Hepatology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984046822102771
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