Journal article
Immunohistochemical Localization of Five Members of the KV1 Channel Subunits: Contrasting Subcellular Locations and Neuron-specific Co-localizations in Rat Brain
The European journal of neuroscience, Vol.7(11), pp.2189-2205
Received 11 July 1994, revised 19 September 1994, accepted 17 May 1995
11/1995
DOI: 10.1111/j.1460-9568.1995.tb00641.x
PMID: 8563969
Abstract
A large variety of potassium channels is involved in regulating integration and transmission of electrical signals in the nervous system. Different types of neurons, therefore, require specific patterns of potassium channel subunits expression and specific regulation of subunit coassembly into heteromultimeric channels, as well as subunit-specific sorting and segregation. This was investigated by studying in detail the expression of six different alpha-subunits of voltage-gated potassium channels in the rat hippocampus, cerebellum, olfactory bulb and spinal cord, combining in situ hybridization and immunocytochemistry. Specific polyclonal antibodies were prepared for five alpha-subunits (Kv1.1, Kv1.2, Kv1.3 Kv1.4, Kv1.6) of the Shaker-related subfamily of rat Kv channels, which encode delayed-rectifier type and rapidly inactivating A-type potassium channels. Their distribution was compared to that of an A-type potassium channel (Kv3.4), belonging to the Shaw-related subfamily of rat Kv channels. Our results show that these Kv channel alpha-subunits are differentially expressed in rat brain neurons. We did not observe in various neurons a stereotypical distribution of Kv channel alpha-subunits to dendritic and axonal compartments, but a complex differential subcellular subunit distribution. The different Kv channel subunits are targeted either to presynaptic or to postsynaptic domains, depending on neuronal cell type. Thus, distinct combinations of Kv1 alpha-subunits are co-localized in different neurons. The implications of these findings are that both differential expression and assembly as well as subcellular targeting of Kv channel alpha-subunits may contribute to Kv channel diversity and thereby to presynaptic and postsynaptic membrane excitability.
Details
- Title: Subtitle
- Immunohistochemical Localization of Five Members of the KV1 Channel Subunits: Contrasting Subcellular Locations and Neuron-specific Co-localizations in Rat Brain
- Creators
- Rüdiger W Veh - Institut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Martinistrasse 52, D-20246 Hamburg, GermanyRalf Lichtinghagen - Institut für Klinische Chemie I, Medizinische Hochschule Hannover, Konstanty-Gutschow-Strasse 8, D-30623 Hannover, GermanySabine Sewing - Institut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Martinistrasse 52, D-20246 Hamburg, GermanyFrank Wunder - Institut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Martinistrasse 52, D-20246 Hamburg, GermanyIsabella M Grumbach - Institut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Martinistrasse 52, D-20246 Hamburg, GermanyOlaf Pongs - Institut für neurale Signalverarbeitung, Zentrum für Molekulare Neurobiologie, Martinistrasse 52, D-20246 Hamburg, Germany
- Resource Type
- Journal article
- Publication Details
- The European journal of neuroscience, Vol.7(11), pp.2189-2205
- Edition
- Received 11 July 1994, revised 19 September 1994, accepted 17 May 1995
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1111/j.1460-9568.1995.tb00641.x
- PMID
- 8563969
- ISSN
- 0953-816X
- eISSN
- 1460-9568
- Number of pages
- 17
- Language
- English
- Date published
- 11/1995
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984094703002771
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