Journal article
Immunohistochemical Markers for Prospective Studies in Neurofibromatosis-1 Porcine Models
The journal of histochemistry and cytochemistry, Vol.65(10), pp.607-618
10/2017
DOI: 10.1369/0022155417729357
PMCID: PMC5624366
PMID: 28846462
Abstract
Neurofibromatosis type 1 (NF1) is a common, cancer-predisposing disease caused by mutations in the NF1 tumor gene. Patients with NF1 have an increased risk for benign and malignant tumors of the nervous system (e.g., neurofibromas, malignant peripheral nerve sheath tumors, gliomas) and other tissues (e.g., leukemias, rhabdomyosarcoma, etc.) as well as increased susceptibility to learning disabilities, chronic pain/migraines, hypertension, pigmentary changes, and developmental lesions (e.g., tibial pseudoarthrosis). Pigs are an attractive and upcoming animal model for future NF1 studies, but a potential limitation to porcine model research has been the lack of validated reagents for direct translational study to humans. To address that issue, we used formalin-fixed tissues (human and pigs) to evaluate select immunohistochemical markers (activated caspase-3, allograft inflammatory factor-1, beta-tubulin III, calbindin D, CD13, CD20, desmin, epithelial membrane antigen, glial fibrillary acidic protein, glucose transporter-1, laminin, myelin basic protein, myoglobin, proliferating cell nuclear antigen, S100, vimentin, and von Willebrand factor). The markers were validated by comparing known expression and localization in human and pig tissues. Validation of these markers on fixed tissues will facilitate prospective immunohistochemical studies of NF1 pigs, as well as other pig models, in a more efficient, reproducible, and translationally relevant manner.
Details
- Title: Subtitle
- Immunohistochemical Markers for Prospective Studies in Neurofibromatosis-1 Porcine Models
- Creators
- David K Meyerholz - Department of PathologyGeorgina K Ofori-Amanfo - Department of PathologyMariah R Leidinger - Department of PathologyJ Adam Goeken - Department of PathologyRajesh Khanna - Departments of Pharmacology and Anesthesiology, College of Medicine, University of Arizona, Tucson, ArizonaJessica C Sieren - Department of Biomedical EngineeringBenjamin W Darbro - Department of PediatricsDawn E Quelle - Department of PharmacologyJill M Weimer - Department of Pediatrics, University of South Dakota, Vermillion, South Dakota
- Resource Type
- Journal article
- Publication Details
- The journal of histochemistry and cytochemistry, Vol.65(10), pp.607-618
- Publisher
- United States
- DOI
- 10.1369/0022155417729357
- PMID
- 28846462
- PMCID
- PMC5624366
- ISSN
- 0022-1554
- eISSN
- 1551-5044
- Grant note
- P30 ES005605 / NIEHS NIH HHS R01 NS082283 / NINDS NIH HHS P01 HL091842 / NHLBI NIH HHS P01 HL051670 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 10/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Stead Family Department of Pediatrics; Pathology; Medical Genetics and Genomics; Neuroscience and Pharmacology
- Record Identifier
- 9984040385202771
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