Journal article
Immunohistochemical Methods for Predicting Cell of Origin and Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With Rituximab
Journal of clinical oncology, Vol.29(2), pp.200-207
01/10/2011
DOI: 10.1200/JCO.2010.30.0368
PMCID: PMC3058275
PMID: 21135273
Abstract
Purpose: Patients with diffuse large B-cell lymphoma (DLBCL) can be divided into prognostic groups based on the cell of origin of the tumor as determined by microarray analysis. Various immunohistochemical algorithms have been developed to replicate these microarray results and/or stratify patients according to survival. This study compares some of those algorithms and also proposes some modifications.
Patients and methods: Two-hundred and sixty-two cases of de novo DLBCL treated with rituximab and cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or CHOP-like therapy were examined.
Results: The Choi algorithm and Hans algorithm had high concordance with the microarray results. Modifications of the Choi and Hans algorithms for ease of use still retained high concordance with the microarray results. Although the Nyman and Muris algorithms had high concordance with the microarray results, each had a low value for either sensitivity or specificity. The use of LMO2 alone showed the lowest concordance with the microarray results. A new algorithm (Tally) using a combination of antibodies, but without regard to the order of examination, showed the greatest concordance with microarray results. All of the algorithms divided patients into groups with significantly different overall and event-free survivals, but with different hazard ratios. With the exception of the Nyman algorithm, this survival prediction was independent of the International Prognostic Index. Although the Muris algorithm had prognostic significance, it misclassified a large number of cases with activated B-cell type DLBCL.
Conclusion: The Tally algorithm showed the best concordance with the microarray data while maintaining prognostic significance and ease of use.
Details
- Title: Subtitle
- Immunohistochemical Methods for Predicting Cell of Origin and Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With Rituximab
- Creators
- Paul N MeyerKAI FUJulie M VoseGeorg LenzLouis M StaudtWing C ChanDennis D WeisenburgerTimothy C GreinerLynette M SmithJan DelabieRandy D GascoyneGerman OttAndreas RosenwaldRita M BrazielElias Campo
- Resource Type
- Journal article
- Publication Details
- Journal of clinical oncology, Vol.29(2), pp.200-207
- DOI
- 10.1200/JCO.2010.30.0368
- PMID
- 21135273
- PMCID
- PMC3058275
- NLM abbreviation
- J Clin Oncol
- ISSN
- 0732-183X
- eISSN
- 1527-7755
- Publisher
- American Society of Clinical Oncology
- Number of pages
- 8
- Language
- English
- Date published
- 01/10/2011
- Academic Unit
- Pathology
- Record Identifier
- 9984822990302771
Metrics
3 Record Views