Journal article
Immunohistochemical demonstration of airway epithelial cell markers of Guinea pig
Tissue & cell, Vol.43(5), pp.283-290
2011
DOI: 10.1016/j.tice.2011.05.003
PMCID: PMC3622455
PMID: 21705035
Abstract
The guinea pig (
Cavea porcellus) is a mammalian non-rodent species in the Caviidae family. The sensitivity of the respiratory system and the susceptibility to infectious diseases allows the guinea pig to be a useful model for both infectious and non-infectious lung diseases such as asthma and tuberculosis. In this report, we demonstrated for the first time, the major cell types and composition in the guinea pig airway epithelium, using cell type-specific markers by immunohistochemical staining using the commercial available immunological reagents that cross-react with guinea pig. Our results revealed the availability of antibodies cross-reacting with airway epithelial cell types of basal, non-ciliated columnar, ciliated, Clara, goblet and alveolar type II cells, as well as those cells expressing Mucin 5AC, Mucin 2, Aquaporin 4 and Calcitonin Gene Related Peptide. The distribution of these various cell types were quantified in the guinea pig airway by immunohistochemical staining and were comparable with morphometric studies using an electron microscopy assay. Moreover, this study also demonstrated that goblet cells are the main secretory cell type in the guinea pig's airway, distinguishing this species from rats and mice. These results provide useful information for the understanding of airway epithelial cell biology and mechanisms of epithelial–immune integration in guinea pig models.
Details
- Title: Subtitle
- Immunohistochemical demonstration of airway epithelial cell markers of Guinea pig
- Creators
- Yong Li - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, ChinaJing Wang - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, ChinaHai Yan He - Department of Pathology, Ningxia People's Hospital, Yinchuan, Ningxia 750021, ChinaLing Jie Ma - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, ChinaJin Zeng - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, ChinaGuang Cun Deng - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, ChinaXiaoming Liu - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242, USAJohn F Engelhardt - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242, USAYujiong Wang - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, Yinchuan, Ningxia 750021, China
- Resource Type
- Journal article
- Publication Details
- Tissue & cell, Vol.43(5), pp.283-290
- DOI
- 10.1016/j.tice.2011.05.003
- PMID
- 21705035
- PMCID
- PMC3622455
- NLM abbreviation
- Tissue Cell
- ISSN
- 0040-8166
- eISSN
- 1532-3072
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2011
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025474402771
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