Journal article
Immunohistochemical detection of arginase-I expression in formalin-fixed lung and other tissues
Journal of histotechnology, Vol.36(4), pp.128-134
12/01/2013
DOI: 10.1179/2046023613Y.0000000032
PMCID: PMC4201120
PMID: 25332513
Abstract
Arginases are a family of enzymes that convert l-arginine to l-ornithine and urea. Alterations in expression of the isoform arginase-I are increasingly recognized in lung diseases such as asthma and cystic fibrosis. To define expression of murine arginase-I in formalin-fixed tissues, including lung, an immunohistochemical protocol was validated in murine liver, a tissue that has distinct zonal arginase-I expression, making it a useful control. In the lung, arginase-I immunostaining was observed in airway surface epithelium and this decreased from large to small airways, with a preferential staining of ciliated epithelium versus Clara cells and alveolar epithelia. In submucosal glands, the ducts and serous acini had moderate immunostaining, which was absent in mucous cells. Focal immunostaining was observed in alveolar macrophages, endothelial cells, pulmonary vein cardiomyocytes, pulmonary artery smooth muscle, airway smooth muscle, and neurons of ganglia of the lung. Arginase-I immunostaining was also detected in other tissues including salivary glands, pancreas, liver, skin, and intestine. Differential immunostaining was observed between sexes in submandibular salivary glands; arginase-I was diffusely expressed in the convoluted granular duct cells of females, but was rarely noted in males. Strain specific differences were not detected. In a mouse with an incidental case of lymphoma, neoplastic lymphocytes lacked arginase-I immunostaining, in contrast to immunostaining detected in non-neoplastic lymphocytes of lymphoid tissues. The use of liver tissue to validate arginase-I immunohistochemistry produced consistent expression patterns in mice, and this approach can be useful to enhance consistency of arginase-I immunohistochemical studies.
Details
- Title: Subtitle
- Immunohistochemical detection of arginase-I expression in formalin-fixed lung and other tissues
- Creators
- Christine M Hochstedler - Iowa Department of Natural ResourcesMariah R Leidinger - Iowa Department of Natural ResourcesMary T Maher-Sturm - Iowa Department of Natural ResourcesKatherine N Gibson-Corley - Iowa Department of Natural ResourcesDavid K Meyerholz - Iowa Department of Natural Resources
- Resource Type
- Journal article
- Publication Details
- Journal of histotechnology, Vol.36(4), pp.128-134
- DOI
- 10.1179/2046023613Y.0000000032
- PMID
- 25332513
- PMCID
- PMC4201120
- NLM abbreviation
- J Histotechnol
- ISSN
- 0147-8885
- eISSN
- 2046-0236
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 12/01/2013
- Academic Unit
- The University of Iowa Institute for Vision Research; Pathology
- Record Identifier
- 9984186530402771
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