Journal article
Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer
Surgery, Vol.161(4), pp.1122-1128
04/01/2017
DOI: 10.1016/j.surg.2016.06.081
PMID: 27919446
Abstract
Background. BRAF(V600E) mutation has been investigated by immunohistochemistry and has shown high sensitivity and specificity. We aim to investigate the accuracy of immunohistochemistry versus molecular testing of BRAF(V600E) in papillary thyroid cancer using a large number of polymerise chain reaction positive BRAF(V600E) papillary thyroid cancer tissues.
Methods. We stained 130 fornzalin-fixed capillary thyroid cancer specimens using the VE1 antibody: 100 BRAF(V600E) positive and 30 BRAF(V600E) negative confirmed by PCR The sensitivity, specificity, and predictive values of the antibody were assessed.
Results. Immunohistochemistry of BRAF(v600E) 98.0% sensitivity, 93.3 % specificity, and positive and negative predictive values of 98.0% and 93.3 %, respectively. Of 100 patients with BRAF(V600E) PCR-positive samples, 97 (97.0%) had cytoplasmic weak (4.0%), moderate (17.0%), and strong (76.0%) immunostaining. In BRAF(V600E) PCR-negative samples, cytoplasmic staining was not detected in 93.3% (28/30) of papillary thyroid cancer tissues. The receiver operating characteristic curve demonstrated a high validity and comparable immunohistochemistry method (area under the curve = 98.8%) compared with PCR testing.
Conclusion. The use of VE1 immunohistochemistry for the detection of BRAF(V600E) in papillary thyroid cancer tissues is a clinically applicable method with high specificity, sensitivity, and positive and negative predictive values. The reliable use of BRAF(V600E) immunohistochemistry should promulgate the routine use of this method for BRAF(V600E) detection in papillary thyroid cancer tissues.
Details
- Title: Subtitle
- Immunohistochemistry as an accurate tool for evaluating BRAF-V600E mutation in 130 samples of papillary thyroid cancer
- Creators
- Zakaria Y. Abd Elmageed - Tulane UniversityAndrew B. Sholl - Tulane UniversityKoji Tsumagari - Tulane UniversityZaid Al-Qurayshi - Tulane UniversityFulvio Basolo - University of PisaKrzysztof Moroz - Tulane UniversityA. Hamid Boulares - Stanley FoundationPaul Friedlander - Tulane UniversityPaulo Miccoli - University of PisaEmad Kandil - Tulane University
- Resource Type
- Journal article
- Publication Details
- Surgery, Vol.161(4), pp.1122-1128
- Publisher
- Elsevier
- DOI
- 10.1016/j.surg.2016.06.081
- PMID
- 27919446
- ISSN
- 0039-6060
- eISSN
- 1532-7361
- Number of pages
- 7
- Grant note
- 1 U54 GM104940 / National Institute of General Medical Sciences of the NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) Tulane University Health Sciences Center U54GM104940 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Language
- English
- Date published
- 04/01/2017
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984702946202771
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