Journal article
Immunohistochemistry for the Novel Markers Glypican 3, PAX8, and p40 (ΔNp63) in Squamous Cell and Urothelial Carcinoma
American journal of clinical pathology, Vol.140(6), pp.872-880
12/01/2013
DOI: 10.1309/AJCP4NSKW5TLGTDS
PMID: 24225756
Abstract
Objectives: To examine squamous cell carcinomas (SCCs) from diverse anatomic sites and invasive urothelial carcinomas (UCs) for expression of the oncofetal antigen glypican 3 (GPC3), the paired box transcription factor PAX8, and the ΔN isoform of p63 (p40).
Methods: Immunohistochemistry for GPC3, PAX8, and p40 was performed on whole sections of 107 SCCs from 11 anatomic sites and 49 UCs; evaluation included extent and intensity of staining.
Results: GPC3 was detected in 20% of SCCs and 12% of UCs and PAX8 in 3% of SCCs, limited to the uterine cervix, and 10% of UCs. p40 Was found in 99% of SCCs and 96% of UCs.
Conclusions: GPC3 expression is frequent in SCC/UC, awareness of which should guard against an incorrect diagnosis of hepatocellular carcinoma, while PAX8, limited in distribution, may have some use in suggesting a cervical or urothelial tract origin in a metastatic squamotransitional carcinoma of unknown primary. There is no drop-off in sensitivity for the diagnoses of SCC or UC with ΔNp63-specific immunohistochemistry, and if this performance can be extended to other applications, p40 may supplant the dominant "pan-p63" antibody clone.
Details
- Title: Subtitle
- Immunohistochemistry for the Novel Markers Glypican 3, PAX8, and p40 (ΔNp63) in Squamous Cell and Urothelial Carcinoma
- Creators
- Michael P Gailey - Department of Pathology, University of Iowa Hospitals and Clinics, University of Iowa Carver College of Medicine, Iowa City, IAAndrew M Bellizzi - Department of Pathology, University of Iowa Hospitals and Clinics, University of Iowa Carver College of Medicine, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- American journal of clinical pathology, Vol.140(6), pp.872-880
- DOI
- 10.1309/AJCP4NSKW5TLGTDS
- PMID
- 24225756
- NLM abbreviation
- Am J Clin Pathol
- ISSN
- 0002-9173
- eISSN
- 1943-7722
- Language
- English
- Date published
- 12/01/2013
- Academic Unit
- Pathology
- Record Identifier
- 9984047854602771
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