Journal article
Immunolocalization and Adenoviral Vector-mediated Manganese Superoxide Dismutase Gene Transfer to Experimental Oral Tumors
Journal of dental research, Vol.79(6), pp.1410-1417
06/2000
DOI: 10.1177/00220345000790061001
PMID: 10890721
Abstract
The anti-oxidant enzyme system protects cellular macromolecules against damage from reactive oxygen species. One component of this system, manganese superoxide dismutase (MnSOD), has also been shown to display tumor suppressor gene-like activity. The purpose of this study was to examine changes in MnSOD expression during hamster cheek pouch carcinogenesis, and the effects of MnSOD overexpression using an adenoviral vector. Tumor induction was carried out using 7,12-dimethylbenz[α]anthracene. Animals were killed at periodic intervals, and check pouch tissues were excised and examined for MnSOD expression by immunohistochemistry and digital image analysis. We observed a reduction in MnSOD expression as early as 2 weeks after the start of carcinogen application. Low MnSOD expression persisted until the end of the 23-week experimental period. Solid hamster cheek pouch carcinoma xenografts were then established in nude mice. An adenoviral vector encoding the human MnSOD gene was delivered to the xenografts by direct injection. We observed high, immediate expression of MnSOD in the xenografts that persisted for 10 days following cessation of viral construct delivery. Delivery of the MnSOD construct resulted in a maximal 50% reduction in tumor growth compared with untreated controls. Our results suggest that MnSOD may be a tumor suppressor gene in the hamster cheek pouch model system.
Details
- Title: Subtitle
- Immunolocalization and Adenoviral Vector-mediated Manganese Superoxide Dismutase Gene Transfer to Experimental Oral Tumors
- Creators
- E.W.N Lam - Department of RadiologyH.M Hammad - Faculty of Dentistry, Jordan University of Science and Technology, Irbid, JordanR Zwacka - MRC Human Genetics Unit, Western General Hospital, Edinburgh, UKC.J Darby - Department of RadiologyK.R Baumgardner - Department of Cariology, Restorative Sciences and Endodontics, University of MichiganB.L Davidson - Department of Internal MedicineT.D Oberley - William S. Middleton Veterans' Memorial Hospital, Madison, WI, USAJ.F Engelhardt - Department of Anatomy and Cell Biology, University of Iowa College of Medicine, Iowa City, IA, USAL.W Oberley - Department of Radiology
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.79(6), pp.1410-1417
- Publisher
- SAGE Publications; Los Angeles, CA
- DOI
- 10.1177/00220345000790061001
- PMID
- 10890721
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Language
- English
- Date published
- 06/2000
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025685202771
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