Journal article
Immunological specificity of novikoff hepatoma chromatin: Isolation of three antigenic proteins
International journal of biochemistry, Vol.14(7), pp.591-600
1982
DOI: 10.1016/0020-711X(82)90042-8
PMID: 7049786
Abstract
1.
1. Immunization of rabbits with dehistonized Novikoff hepatoma or normal rat liver chromatin elicited specific antibodies.
2.
2. Chromosomal nonhistone proteins from Novikoff hepatoma were fractionated and three proteins (approx. mol. wt 39,000, 49,000 and 56,000) were identified with the hepatoma antigenic complexes.
3.
3. All three proteins showed significant reactivity with PASt staining suggesting that they were glycosylated.
4.
4. Proteins migrating similar to these three Novikoff hepatoma fractions were detected by polyacrylamide gel electrophoresis in the chromosomal nonhistone protein fraction obtained from normal rat liver.
5.
5. However, the three proteins from normal liver were PAS negative, and did not react immunologically with antiserum to dehistonized Novikoff hepatoma chromatin.
Details
- Title: Subtitle
- Immunological specificity of novikoff hepatoma chromatin: Isolation of three antigenic proteins
- Creators
- Marta Stryjecka-ZimmerWarren N SchmidtRobert C BriggsLubomir S Hnilica
- Resource Type
- Journal article
- Publication Details
- International journal of biochemistry, Vol.14(7), pp.591-600
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0020-711X(82)90042-8
- PMID
- 7049786
- ISSN
- 0020-711X
- Language
- English
- Date published
- 1982
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094627602771
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