Journal article
Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice
Molecular & cellular proteomics, Vol.23(9), 100829
09/2024
DOI: 10.1016/j.mcpro.2024.100829
PMCID: PMC11414675
PMID: 39147027
Abstract
Listeria monocytogenes is a foodborne intracellular bacterial model pathogen. Protective immunity against Listeria depends on an effective CD8+ T cell response, but very few T cell epitopes are known in mice as a common animal infection model for listeriosis. To identify epitopes we screened for Listeria immunopeptides presented in the spleen of infected mice by mass spectrometry-based immunopeptidomics. We mapped more than 6,000 mouse self-peptides presented on MHC Class I molecules, including 12 high confident Listeria peptides from 12 different bacterial proteins. Bacterial immunopeptides with confirmed fragmentation spectra were further tested for their potential to activate CD8+ T cells, revealing VTYNYINI from the putative cell wall surface anchor family protein LMON_0576 as a novel bona fide peptide epitope. The epitope showed high biological potency in a prime boost model and can be used as a research tool to probe CD8+ T cell responses in mouse models of Listeria infection. Together, our results demonstrate the power of immunopeptidomics for bacterial antigen identification.
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•Identification of >6,000 MHC Class I immunopeptides in Listeria-infected mice.•High-confident detection of 12 Listeria-derived immunopeptides.•Discovery of VTYNYINI as a potent Listeria CD8+ T cell epitope.
Details
- Title: Subtitle
- Immunopeptidomics mapping of Listeria monocytogenes T cell epitopes in mice
- Creators
- Adillah Gul - VIB-UGent Center for Medical BiotechnologyLecia L. Pewe - University of IowaPatrick Willems - VIB-UGent Center for Medical BiotechnologyRupert Mayer - VIB-UGent Center for Medical BiotechnologyFabien Thery - VIB-UGent Center for Medical BiotechnologyCaroline Asselman - VIB-UGent Center for Medical BiotechnologyIlke Aernout - Cancer Research Institute GhentRein Verbeke - Cancer Research Institute GhentDenzel Eggermont - VIB-UGent Center for Medical BiotechnologyLaura Van Moortel - VIB-UGent Center for Medical BiotechnologyEllen Upton - University of IowaYifeng Zhang - University of IowaKatie Boucher - VIB-UGent Center for Medical BiotechnologyLaia Miret-Casals - VIB-UGent Center for Medical BiotechnologyHans Demol - VIB-UGent Center for Medical BiotechnologyStefaan C. De Smedt - Ghent UniversityIne Lentacker - Ghent UniversityLilliana Radoshevich - University of IowaJohn T. Harty - University of IowaFrancis Impens - VIB-UGent Center for Medical Biotechnology
- Resource Type
- Journal article
- Publication Details
- Molecular & cellular proteomics, Vol.23(9), 100829
- DOI
- 10.1016/j.mcpro.2024.100829
- PMID
- 39147027
- PMCID
- PMC11414675
- NLM abbreviation
- Mol Cell Proteomics
- ISSN
- 1535-9476
- eISSN
- 1535-9484
- Publisher
- Elsevier Inc
- Grant note
- Research Foundation-Flanders (FWO): G0F8616N, 12AN524N, 12T1722N, 1275023N, 1S40923N, 1167223N Ghent University Concerted Research Action grant: BOF21/GOA/033 Horizon Europe Project BAXERNA 2.0: 101080544 NIGMS: R35GM137961 NIH/NIAID: AI114543, AI178159, AI167847, AI42767
R. L. M. acknowledges support from the Flemish Institute for Biotechnology (VIB) and a faculty interim fellowship from the UGent Faculty of Medicine and Health Sciences. This work was supported by the Research Foundation-Flanders (FWO) by Odysseus grant G0F8616N to F. I. and fellowships to F. T. (12AN524N), P. W. (12T1722N), R. V. (1275023N), I.A. (1S40923N), and D. E. (1167223N). A. G. is supported by a PhD fellowship from the Higher Education Commission (HEC) Pakistan. F. I. and I. L. acknowledge support from Ghent University Concerted Research Action grant BOF21/GOA/033 and the Horizon Europe Project BAXERNA 2.0 (101080544). L. R. is supported by NIGMS grant R35GM137961 and by a University of Iowa Stead Fellowship. J. T. H. was supported by NIH/NIAID grants AI114543, AI178159, AI167847, AI42767.
- Language
- English
- Electronic publication date
- 08/13/2024
- Date published
- 09/2024
- Academic Unit
- Molecular Physiology and Biophysics; Pathology
- Record Identifier
- 9984696864402771
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