Journal article
Immunoprecipitable polypeptides specified by varicella-zoster virus
Virology (New York, N.Y.), Vol.118(1), pp.86-95
1982
DOI: 10.1016/0042-6822(82)90322-1
PMID: 6177095
Abstract
Polypeptides encoded by varicella-zoster virus (VZV) in infected cell cultures have been identified by radioimmune precipitation techniques. Detergent-solubilized extracts of VZV-infected cells were reacted with highly specific VZV antisera raised in strain-2 guinea pigs immunized with sonicates of syngeneic virus-infected cells. Fractionation of the immunoprecipitates in acrylamide slab gels demonstrated an average of 16 polypeptides, which ranged in molecular weight from 32,000 to å200,000. These included the three major immunogenic glycoproteins (gp 62, gp 98, and gp 118) and a prominent higher molecular weight nonglycosylated polypeptide at 155,000. One of the [
35S]methionine-labeled polypeptides comigrated with purified actin. Not all polypeptides were visible in any one particular fluorogram, but comparative analysis of polypeptide profiles derived from electrophoreses performed with different gel concentrations and different crosslinkers (methylene-bisacrylamide and
N,N-diallyltartardiamide) clearly established a consistent and reproducible pattern of radioactive bands. A low background of radio-activity was nonspecifically precipitated by the antigen-antibody-protein A complexes; however, with the exception of a common band comigrating with actin, the electrophoretic profiles representing virus-specific and nonspecific immunoprecipitates were easily distinguished.
Details
- Title: Subtitle
- Immunoprecipitable polypeptides specified by varicella-zoster virus
- Creators
- Charles GroseWilliam E Friedrichs
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.118(1), pp.86-95
- DOI
- 10.1016/0042-6822(82)90322-1
- PMID
- 6177095
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1982
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093463002771
Metrics
14 Record Views