Journal article
Immunostaining to identify molecular subtypes of diffuse large B-cell lymphoma in a population-based epidemiologic study in the pre-rituximab era
International journal of molecular epidemiology and genetics, Vol.2(3), pp.245-252
08/30/2011
PMCID: PMC3166152
PMID: 21915363
Abstract
Gene expression profiling studies have distinguished diffuse large B-cell lymphomas (DLBCLs) by cell of origin, with distinct pathogenetic mechanisms and prognosis. We attempted to identify DLBCL molecular subtypes in an epidemiologic study of 214 DLBCL patients diagnosed during 1998-2000 with archival tissues to investigate etiology. Immunohistochemical staining for CD10, BCL6, LMO2, MUM1/IRF4, and BCL2 and fluorescence
in situ
hybridization for t(14;18) were conducted, with ≥93% blinded duplicate agreement. CD10, LMO2, and BCL2 expression was similar to previous reports (32%, 44%, and 44% of DLBCLs, respectively), but BCL6 and MUM1/IRF4 expression was lower than expected (29% and 5%, respectively). We classified 112/214 (52%) cases as germinal center B-cell-like DLBCL (GCB-DLBCL; Hans
et al., Blood
2004; CD10+ or CD10-/BCL6+/MUM1-), with no difference in prognosis compared with non-GCB-DLBCL (Cox regression, P=0.48). Comparing other GCB correlates, LMO2 expression and t(14;18) were more common but not exclusive to GCB-DLBCL as defined in our study, whereas BCL2 expression did not differ between DLBCL molecular subtypes. We could not confidently identify patients with GCB-DLBCL using these immunohistochemistry-based markers on archival tissues.
Details
- Title: Subtitle
- Immunostaining to identify molecular subtypes of diffuse large B-cell lymphoma in a population-based epidemiologic study in the pre-rituximab era
- Creators
- Lindsay M Morton - Division of Cancer Epidemiology and Genetics, National Cancer InstituteJames R Cerhan - Mayo Clinic College of MedicinePatricia Hartge - Division of Cancer Epidemiology and Genetics, National Cancer InstituteMohammad A Vasef - Department of Pathology, University of New MexicoVishala T Neppalli - Depart-ment of Pathology, University of IowaYasodha Natkunam - Department of Pathology, Stanford University School of MedicineAhmet Dogan - Mayo Clinic College of MedicineBhavana J Dave - University of Nebraska Medical CenterSmrati Jain - University of Nebraska Medical CenterRonald Levy - Division of Oncology, Stanford University School of MedicineIzidore S Lossos - Department of Medicine, Division of Hematology/Oncology, University of Miami/ Sylvester Comprehensive Cancer CenterWendy Cozen - Keck School of Medicine, University of Southern CaliforniaScott Davis - Fred Hutchinson Cancer Research Center and University of WashingtonMary Jean Schenk - Barbara Ann Karmanos Cancer Institute, Wayne State UniversityMatthew J Maurer - Mayo Clinic College of MedicineCharles F Lynch - Department of Epidemiology, University of IowaNathaniel Rothman - Division of Cancer Epidemiology and Genetics, National Cancer InstituteNilanjan Chatterjee - Division of Cancer Epidemiology and Genetics, National Cancer InstituteKai Yu - Division of Cancer Epidemiology and Genetics, National Cancer InstituteLouis M Staudt - Center for Cancer Research, National Cancer InstituteDennis D Weisenburger - University of Nebraska Medical CenterSophia S Wang - Division of Etiology, Department of Population Sciences
- Resource Type
- Journal article
- Publication Details
- International journal of molecular epidemiology and genetics, Vol.2(3), pp.245-252
- Publisher
- e-Century Publishing Corporation
- PMID
- 21915363
- PMCID
- PMC3166152
- eISSN
- 1948-1756
- Language
- English
- Date published
- 08/30/2011
- Academic Unit
- Epidemiology
- Record Identifier
- 9983995120402771
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