Immunotoxicity of explosives-contaminated soil before and after bioremediation

L. A BELTZ; D. R NEIRA; C. A AXTELL; S IVERSON; W DEATON; T. J WALDSCHMIDT; J. A BUMPUS; C. G JOHNSTON

doi:10.1007/s002440010177

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Immunotoxicity of explosives-contaminated soil before and after bioremediation

Journal article

Peer reviewed

Immunotoxicity of explosives-contaminated soil before and after bioremediation

L. A BELTZ, D. R NEIRA, C. A AXTELL, S IVERSON, W DEATON, T. J WALDSCHMIDT, J. A BUMPUS and C. G JOHNSTON

Archives of environmental contamination and toxicology, Vol.40(3), pp.311-317

2001

DOI: 10.1007/s002440010177

PMID: 11443360

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Abstract

Soils from the Yorktown Naval Base contaminated with trinitrotoluene (TNT) and other explosives were used to prepare eluates before and after bioremediation using microbial growth amendments in the presence (P1 eluates) or absence (P2 eluates) of exogenous white rot fungus. Effectiveness of bioremediation was examined by several immunotoxicity assays-viability/growth of lymphocytes, cytokine production, and expression of the interleukin-2 (IL-2) receptor-using human peripheral blood mononuclear cells exposed to the eluates. Although TNT concentrations decreased in both P1 and P2 eluates relative to untreated baseline soil (BL) eluates, a recovery in lymphocyte growth/viability and IL-2 secretion was seen with P2 but not P1 eluates relative to BL eluates. IL-2 receptor levels were higher in cells exposed to BL and P2 eluates than when exposed to P1 eluates. Interferon-gamma, tumor necrosis factor-beta, and IL-10 levels were highest in BL and P2 eluates and lowest in P1 eluates. Taken together, these results suggest that treatment of the soil with microbial growth amendments in the absence but not the presence of exogenous white rot fungi lead to partial bioremediation as assessed by lymphocyte functions.

Biotechnology

Fundamental and applied biological sciences. Psychology

Biodegradation of pollutants

Terrestrial environment, soil, air

Animal, plant and microbial ecology

Industrial applications and implications. Economical aspects

Biological and medical sciences

Environment and pollution

Applied ecology

Ecotoxicology, biological effects of pollution

Details

Title: Subtitle: Immunotoxicity of explosives-contaminated soil before and after bioremediation
Creators: L. A BELTZ - Department of Biology, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
D. R NEIRA - Department of Biology, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
C. A AXTELL - Environmental Programs, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
S IVERSON - Department of Biology, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
W DEATON - Department of Biology, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
T. J WALDSCHMIDT - Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, United States
J. A BUMPUS - Environmental Programs, University of Northern Iowa, Cedar Falls, Iowa 50614, United States
C. G JOHNSTON - Mycotech Corporation, Butte, Montana, United States
Resource Type: Journal article
Publication Details: Archives of environmental contamination and toxicology, Vol.40(3), pp.311-317
Publisher: Springer-Verlag; Heidelberg; Berlin; New York, NY
DOI: 10.1007/s002440010177
PMID: 11443360
ISSN: 0090-4341
eISSN: 1432-0703
Language: English
Date published: 2001
Academic Unit: Pathology
Record Identifier: 9984046806202771

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