Impact of aging on rat bone marrow-derived stem cell chondrogenesis

Hongjun Zheng; James A Martin; Yazan Duwayri; Gilbert Falcon; Joseph A Buckwalter

doi:10.1093/gerona/62.2.136

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Impact of aging on rat bone marrow-derived stem cell chondrogenesis

Journal article

Open access

Peer reviewed

Impact of aging on rat bone marrow-derived stem cell chondrogenesis

Hongjun Zheng, James A Martin, Yazan Duwayri, Gilbert Falcon and Joseph A Buckwalter

The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.62(2), pp.136-148

02/2007

DOI: 10.1093/gerona/62.2.136

PMID: 17339639

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https://doi.org/10.1093/gerona/62.2.136View

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Abstract

Damaged articular cartilage rarely heals or regenerates in middle-aged and elderly adults, suggesting that the chondrogenic potential of mesenchymal stem cells declines with age. To test this hypothesis, we measured the responses of rat bone marrow-derived mesenchymal stem cells (BMSCs) to chondrogenic induction in vitro. BMSCs from immature rats (1 week old), young adult rats (12 weeks old), and old adult rats (1 year old) were analyzed for cartilage extracellular matrix (ECM) production. Histologic analysis showed strong cartilage ECM formation by BMSCs from 1-week-old rats, but not by BMSCs from 12-week-old or 1-year-old rats. Real-time polymerase chain reaction revealed age-related declines in messenger RNA encoding type II collagen, aggrecan, and link protein, three major cartilage ECM components. Microarray analysis indicated significant age-related differences in the expression of genes that influence cartilage ECM formation. These findings support the hypothesis that the chondrogenic potential of mesenchymal stem cells declines with age.

Cell Proliferation

Extracellular Matrix

Proteoglycans - metabolism

Rats

Male

Procollagen - metabolism

Aggrecans - metabolism

RNA, Messenger - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Rats, Sprague-Dawley

Mesenchymal Stem Cells

Collagen Type II - metabolism

Cartilage

Animals

Bone Marrow Cells

Microarray Analysis

Fluorescent Antibody Technique

Aging

Models, Animal

Chondrogenesis

In Vitro Techniques

Chondrocytes - metabolism

Extracellular Matrix Proteins - metabolism

Details

Title: Subtitle: Impact of aging on rat bone marrow-derived stem cell chondrogenesis
Creators: Hongjun Zheng - Department of Orthopaedics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
James A Martin
Yazan Duwayri
Gilbert Falcon
Joseph A Buckwalter
Resource Type: Journal article
Publication Details: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.62(2), pp.136-148
DOI: 10.1093/gerona/62.2.136
PMID: 17339639
NLM abbreviation: J Gerontol A Biol Sci Med Sci
ISSN: 1079-5006
eISSN: 1758-535X
Publisher: United States
Grant note: P50 AR48939 / NIAMS NIH HHS
Language: English
Date published: 02/2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
Record Identifier: 9984040572802771

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