Journal article
Impact of interleukin 13 (IL13) genetic polymorphism Arg130Gln on total serum immunoglobulin (IgE) levels and interferon (IFN)- gene expression
Clinical and experimental immunology, Vol.188(1), pp.45-52
04/01/2017
DOI: 10.1111/cei.12923
PMCID: PMC5343340
PMID: 28054352
Abstract
This cross-sectional study was designed to investigate the extent of genetic susceptibility by targeting variants in interleukin (IL)-4/IL-13 signalling pathways leading to atopic disease in early childhood. We evaluated involvement of five single nucleotide polymorphisms IL4 C-590T, IL13 C-1055T, IL13 Arg130Gln, IL4RA Ile50Val and IL4RA Gln576Arg, in the control of serum total and antigen-specific immunoglobulin (Ig)E levels. Furthermore, we analysed their association with changes in gene expression of five cytokines having key roles in inflammatory and anti-inflammatory immune response [IL-4, IL-13, interferon (IFN)-, IL-8 and IL-10]. Total and antigen-specific IgE levels in serum and gene expression of selected cytokines in peripheral blood were measured in 386 children aged 1-8 years. TaqMan allelic discrimination, amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and restriction fragment length polymorphisms (RFLP) methods validated by sequencing were used for genotyping. All genotypes for children with total and antigen-specific IgE levels in the normal range were in Hardy-Weinberg equilibrium. Gene expression analyses were carried out using TaqMan gene expression assays. We found elevated total IgE levels in carriers of IL13 Arg130Gln variant allele [odds ratio (OR) =184; 95% confidence interval (CI)=116-293]. This effect was more apparent for boys (OR=231; 95% CI=125-428). However, no significant association was observed for the other four variants examined. We found up-regulation of IFN- in children with elevated serum total IgE levels carrying the Arg130 allele (P=0005). No differences were found for IL4, IL8 or IL10, while IL13 gene expression was under the detection limit. IL13 Arg130Gln genotypes can play a role in genetic susceptibility to allergy via regulation of serum total IgE levels and affecting IFN- gene expression.
Details
- Title: Subtitle
- Impact of interleukin 13 (IL13) genetic polymorphism Arg130Gln on total serum immunoglobulin (IgE) levels and interferon (IFN)- gene expression
- Creators
- B. Smolkova - Slovak Academy of SciencesJ. Tulinska - Slovak Medical UniversityL. Palkovicova Murinova - Slovak Medical UniversityV. Buocikova - Slovak Medical UniversityA. Liskova - Slovak Medical UniversityK. Rausova - Slovak Medical UniversityM. Kuricova - Slovak Medical UniversityH. Patayova - Slovak Medical UniversityM. Sustrova - Slovak Medical UniversityE. Neubauerova Svorcova - Slovak Medical UniversityS. Ilavska - Slovak Medical UniversityM. Szabova - Slovak Medical UniversityT. Nemessanyi - Slovak Medical UniversityE. Jahnova - Slovak Medical UniversityM. Dusinska - Norwegian Institute for Air ResearchP. Ciznar - Comenius University BratislavaL. Fuortes - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Clinical and experimental immunology, Vol.188(1), pp.45-52
- Publisher
- Wiley
- DOI
- 10.1111/cei.12923
- PMID
- 28054352
- PMCID
- PMC5343340
- ISSN
- 0009-9104
- eISSN
- 1365-2249
- Number of pages
- 8
- Grant note
- 2 D43 TW00621-006 / USA NIEHS APVT-21-016504 / Slovak Research and Development Agency European Regional Development Fund; European Commission FOODCT-2005-016320 / EC; European Commission; European Commission Joint Research Centre 2005/40-SZU-18 / Ministry of Health of the Slovak Republic QLK4-CT-2000-00279 / EU; European Commission 26240120033 / ITMS D43TW000621 / FOGARTY INTERNATIONAL CENTER; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Fogarty International Center (FIC)
- Language
- English
- Date published
- 04/01/2017
- Academic Unit
- Occupational and Environmental Health; Internal Medicine
- Record Identifier
- 9984363605802771
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