Journal article
Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome
Molecular and biochemical parasitology, Vol.147(2), pp.163-176
06/01/2006
DOI: 10.1016/j.molbiopara.2006.02.007
PMID: 16569449
Abstract
A genome-wide transcriptional analysis of
Entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. An Affymetrix platform gene expression array was designed for this analysis that included probe sets for 9435 open reading frames (ORFs) and 9066 5′ and 3′ flanking regions. Transcripts were detected for >80% of all ORFs. A total of 523 transcripts (5.2% of all
E. histolytica genes) were significantly changed in amebae isolated from the intestine on Days 1 and 29 after infection: 326 and 109 solely on Days 1 and 29, and 88 on both days. Quantitative real-time reverse transcriptase PCR confirmed these changes in 11/12 genes tested using mRNA isolated from an additional six mice. Adaptation to the intestinal environment was accompanied by increases in a subset of cell signaling genes including transmembrane kinases, ras and rho family GTPases, and calcium binding proteins. Significant decreases in mRNA abundance for genes involved in glycolysis and concomitant increases in lipases were consistent with a change in energy metabolism. Defense against bacteria present in the intestine (but lacking from in vitro culture) was suggested by alterations in mRNA levels of genes similar to the AIG1 plant antibacterial proteins. Decreases in oxygen detoxification pathways were observed as expected in the anaerobic colonic lumen. Of the known virulence factors the most remarkable changes were a 20–35-fold increase in a cysteine proteinase four-like gene, and a 2–3-fold decrease in two members of the Gal/GalNAc lectin light subunit family. Control of the observed changes in mRNA abundance in the intestine might potentially rest with four related proteins with DNA binding domains that were down-regulated 6–16-fold in the intestinal environment. In conclusion, the first genome-wide analysis of the transcriptome of
E. histolytica demonstrated that the vast majority of genes are transcribed in trophozoites, and that in the host intestine trophozoites altered the expression of mRNAs for genes implicated in metabolism, oxygen defense, cell signaling, virulence, antibacterial activity, and DNA binding.
Details
- Title: Subtitle
- Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome
- Creators
- Carol A. Gilchrist - University of VirginiaEric Houpt - University of VirginiaNino Trapaidze - National Center for Disease Control and Public HealthZhangjun Fei - Virginia TechOswald Crasta - Virginia TechAmon Asgharpour - University of VirginiaClive Evans - Virginia TechSusan Martino-Catt - Virginia TechDuza J. Baba - University of VirginiaSuzanne Stroup - University of VirginiaShinjiro Hamano - University of VirginiaGretchen Ehrenkaufer - Stanford MedicineMami Okada - Gunma UniversityUpinder Singh - Stanford MedicineTomoyoshi Nozaki - Gunma UniversityBarbara J. Mann - University of VirginiaWilliam A. Petri - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.147(2), pp.163-176
- DOI
- 10.1016/j.molbiopara.2006.02.007
- PMID
- 16569449
- NLM abbreviation
- Mol Biochem Parasitol
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Publisher
- Elsevier B.V
- Number of pages
- 14
- Language
- English
- Date published
- 06/01/2006
- Academic Unit
- Internal Medicine
- Record Identifier
- 9985244667602771
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