Journal article
Impaired Assembly yet Normal Trafficking of MHC Class I Molecules in Tapasin Mutant Mice
Immunity (Cambridge, Mass.), Vol.13(2), pp.213-222
2000
DOI: 10.1016/S1074-7613(00)00021-2
PMID: 10981964
Abstract
Loading of peptides onto major histocompatibility complex class I molecules involves a multifactorial complex that includes tapasin (TPN), a membrane protein that tethers empty class I glycoproteins to the transporter associated with antigen processing. To evaluate the in vivo role of TPN, we have generated
Tpn mutant mice. In these animals, most class I molecules exit the endoplasmic reticulum (ER) in the absence of stably bound peptides. Consequently, mutant animals have defects in class I cell surface expression, antigen presentation, CD8
+ T cell development, and immune responses. These findings reveal a critical role of TPN for ER retention of empty class I molecules.
Tpn mutant animals should prove useful for studies on alternative antigen-processing pathways that involve post-ER peptide loading.
Details
- Title: Subtitle
- Impaired Assembly yet Normal Trafficking of MHC Class I Molecules in Tapasin Mutant Mice
- Creators
- Andres G Grandea - Department of Microbiology and Immunology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USATatiana N Golovina - Department of Microbiology and Immunology, Jefferson Medical College and, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USASara E Hamilton - The Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USAVenkataraman Sriram - Department of Microbiology and Immunology, Indiana University School of Medicine, Walther Oncology Center, Indianapolis, Indiana 46202, USAThomas Spies - Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USARandy R Brutkiewicz - Department of Microbiology and Immunology, Indiana University School of Medicine, Walther Oncology Center, Indianapolis, Indiana 46202, USAJohn T Harty - The Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USALaurence C Eisenlohr - Department of Microbiology and Immunology, Jefferson Medical College and, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USALuc Van Kaer - Department of Microbiology and Immunology, Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
- Resource Type
- Journal article
- Publication Details
- Immunity (Cambridge, Mass.), Vol.13(2), pp.213-222
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S1074-7613(00)00021-2
- PMID
- 10981964
- ISSN
- 1074-7613
- eISSN
- 1097-4180
- Language
- English
- Date published
- 2000
- Academic Unit
- Pathology
- Record Identifier
- 9984046830302771
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