Journal article
Impaired class II expression and antigen uptake in monocytic cells after HIV-1 infection
The Journal of immunology (1950), Vol.159(5), pp.2177-2188
09/01/1997
DOI: 10.4049/jimmunol.159.5.2177
PMID: 9278305
Abstract
Using the human macrophage hybridoma cell line 43 and primary monocytes, we investigated the regulation of class II expression and intracellular Ag trafficking after HIV-1 infection. The HIV-1-infected human macrophage hybridoma cell line, 43HIV, lost class II Ag expression, as determined by immunofluorescence, immunoprecipitation, and Northern blot analysis, 2 wk after infection. Class II expression could be restored by transfection with the full-length HLA-DR4 cDNA driven by a CMV IE promotor. However, even after transfection, the 43HIV cells were incapable of presenting Ag to MHC-matched Ag-specific T cells. This defect was associated with decreased formation of class II-Ag complexes, and similar findings were observed in primary HIV-1BaL-infected monocytes. We investigated Ag uptake using FITC-labeled tetanus, OVA, and keyhole limpet hemocyanin. There was decreased uptake of all three Ags after HIV-1 infection at different time points after Ag pulsing in the 43HIV cells and in primary HIV-1BaL-infected monocytes. There was colocalization of the FITC-labeled Ags with early (cathepsin D) and late endosomal markers (anti-mannose-6-phosphate receptor), lysosomal markers (CD-63), and acidic compartment markers (3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine) in the uninfected cells, but the level of colocalized Ag was reduced in the 43HIV cells and HIV-1BaL-infected monocytes. Our data suggest that class II expression, formation of class II-Ag complexes, and Ag uptake are impaired in chronically HIV-1-infected monocytic cells, which may contribute to the global immunosuppression observed in AIDS.
Details
- Title: Subtitle
- Impaired class II expression and antigen uptake in monocytic cells after HIV-1 infection
- Creators
- Steven Polyak - Division of Clinical Immunology, Mount Sinai Medical Center, New York, NY 10029, USAHouchu ChenDavid HirschItalas GeorgeRobert HershbergKirk Sperber
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.159(5), pp.2177-2188
- DOI
- 10.4049/jimmunol.159.5.2177
- PMID
- 9278305
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- CAR-29-256990 / NCI NIH HHS
- Language
- English
- Date published
- 09/01/1997
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984094601102771
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