Journal article
Impaired melanocortin pathway function in Prader-Willi syndrome gene-Magel2 deficient mice
Human molecular genetics, Vol.27(18), pp.3129-3136
09/15/2018
DOI: 10.1093/hmg/ddy216
PMID: 29878108
Abstract
Abstract
Prader-Willi Syndrome (PWS) is a neurodevelopmental disorder causing social and learning deficits, impaired satiety and severe childhood obesity. Genetic underpinning of PWS involves deletion of a chromosomal region with several genes, including MAGEL2, which is abundantly expressed in the hypothalamus. Of appetite regulating hypothalamic cell types, both AGRP and POMC-expressing neurons contain Magel2 transcripts but the functional impact of its deletion on these cells has not been fully characterized. Here, we investigated these key neurons in Magel2-null mice in terms of the activity levels at different energy states as well as their behavioral function. Using cell type specific ex vivo electrophysiological recordings and in vivo chemogenetic activation approaches we evaluated impact of Magel2 deletion on AGRP and POMC-neuron induced changes in appetite. Our results suggest that POMC neuron activity profile as well as its communication with downstream targets is significantly compromised, while AGRP neuron function with respect to short term feeding is relatively unaffected in Magel2 deficiency.
Details
- Title: Subtitle
- Impaired melanocortin pathway function in Prader-Willi syndrome gene-Magel2 deficient mice
- Creators
- Merve Oncul - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyPelin Dilsiz - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyEdanur Ates Oz - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyTayfun Ates - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyIltan Aklan - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyEsref Celik - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyNilufer Sayar Atasoy - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, TurkeyDeniz Atasoy - Department of Physiology, School of Medicine, Regenerative and Restorative Medical Research Center (REMER), Istanbul Medipol University, Istanbul, Turkey
- Resource Type
- Journal article
- Publication Details
- Human molecular genetics, Vol.27(18), pp.3129-3136
- Publisher
- Oxford University Press
- DOI
- 10.1093/hmg/ddy216
- PMID
- 29878108
- ISSN
- 0964-6906
- eISSN
- 1460-2083
- Grant note
- 2539 / European Molecular Biology Organization (10.13039/100004410)
- Language
- English
- Date published
- 09/15/2018
- Academic Unit
- Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
- Record Identifier
- 9984040279202771
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