Impairment of endothelium-dependent vasodilation induced by acetylcholine and adenosine triphosphate following experimental subarachnoid hemorrhage

Tadayoshi Nakagomi; Neal F Kassell; Tomio Sasaki; Shigeru Fujiwara; R Michael Lehman; James C Torner

doi:10.1161/01.STR.18.2.482

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Impairment of endothelium-dependent vasodilation induced by acetylcholine and adenosine triphosphate following experimental subarachnoid hemorrhage

Journal article

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Impairment of endothelium-dependent vasodilation induced by acetylcholine and adenosine triphosphate following experimental subarachnoid hemorrhage

Tadayoshi Nakagomi, Neal F Kassell, Tomio Sasaki, Shigeru Fujiwara, R Michael Lehman and James C Torner

Stroke (1970), Vol.18(2), pp.482-489

03/1987

DOI: 10.1161/01.STR.18.2.482

PMID: 3564107

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Abstract

The effect of subarachnoid hemorrhage (SAH) on endothelium-dependent vasodilation of isolated rabbit basilar artery was examined using an isometric tension recording method. Thirty-five rabbits that had 2 successive blood injections were divided into 3 groups: normal animals (control), 4 days, and 3 weeks after the first SAH. Acetylcholine (ACh) (10(-6)-10(-4) M) and adenosine triphosphate (ATP) (10(-6)-10(-4) M) were used to evoke dose-dependent vasodilation of isolated arterial rings previously contracted by 10(-6) M serotonin. In the animals killed 4 days after the first SAH, both ACh- and ATP-induced relaxation were suppressed, and the degree of relaxation of this group was 38 +/- 4.5% (mean +/- SEM) and 22 +/- 3.9% of the initial contractile tone in response to 10(-4) M ACh and 10(-4) M ATP, respectively. Suppression of the relaxation induced by ATP was seen even in the animals killed 3 weeks after the first SAH. Moreover, pretreatment with hemoglobin (10(-6) and 10(-5) M) inhibited endothelium-dependent vasodilation induced by ACh in the arterial rings from the animals killed 4 days after the first SAH. The present experiments suggest that impairment of the endothelium-dependent vasodilation following SAH may be involved in the pathogenesis of cerebral vasospasm.

Adenosine Triphosphate - pharmacology

Rabbits

Subarachnoid Hemorrhage - physiopathology

Animals

Acetylcholine - pharmacology

Basilar Artery - pathology

Cerebral Arteries - physiopathology

Male

Hemoglobins - pharmacology

Vasodilation - drug effects

Subarachnoid Hemorrhage - pathology

Endothelium - physiopathology

Details

Title: Subtitle: Impairment of endothelium-dependent vasodilation induced by acetylcholine and adenosine triphosphate following experimental subarachnoid hemorrhage
Creators: Tadayoshi Nakagomi
Neal F Kassell
Tomio Sasaki
Shigeru Fujiwara
R Michael Lehman
James C Torner
Resource Type: Journal article
Publication Details: Stroke (1970), Vol.18(2), pp.482-489
DOI: 10.1161/01.STR.18.2.482
PMID: 3564107
NLM abbreviation: Stroke
ISSN: 0039-2499
eISSN: 1524-4628
Publisher: United States
Language: English
Date published: 03/1987
Academic Unit: Epidemiology; Surgery; Injury Prevention Research Center; Neurosurgery
Record Identifier: 9983996082202771

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