Journal article
Impairment of the GABAergic system in the anterior midcingulate cortex of heroin-addicted males
European archives of psychiatry and clinical neuroscience, Vol.275(7), pp.2111-2118
10/2025
DOI: 10.1007/s00406-025-01992-3
PMID: 40116918
Abstract
Opioid addiction is a global concern and the largest health burden among drug use disorders. The multifunctional anterior midcingulate cortex (aMCC) is critical for processing nociceptive input and negative emotions, which play a prominent role in the maintenance of opioid addiction. GABAergic interneurons regulate the output of the aMCC, whose dysfunction has been linked to the behavioural abnormalities observed in addiction. In these neurons, glutamate decarboxylase (GAD), with its isoforms GAD 65 and 67, is a key enzyme in the synthesis of GABA. However, there is a lack of research investigating the role of the GABAergic system in the aMCC in the context of opioid addiction. Our study, which was performed on paraffin-embedded brains from the Magdeburg Brain Bank, aimed to investigate abnormalities in the GABAergic function of the aMCC in opioid addiction by densitometric evaluation of GAD 65/67-immunostained neuropil. Assessment of neuropil was performed in parallel with assessment of density of immunostained somata. The study showed decreased neuropil density in layers III and V in the left MCC of 13 heroin-addicted males compared to 12 healthy controls, with a significant U-test P value for layer V. In contrast, the density of GAD-immunostained somata was increased bilaterally in MCC layers III and V, but not significantly. Analysis of confounding variables showed that age, brain volume and duration of formalin fixation did not confound the results. Our findings are the first to suggest a dysregulation of GABAergic system in the aMCC in opioid-addicted individuals, contributing to the understanding of opioid addiction.
Details
- Title: Subtitle
- Impairment of the GABAergic system in the anterior midcingulate cortex of heroin-addicted males
- Creators
- Apollonia von Gilsa - Otto-von-Guericke University MagdeburgJohann Steiner - Otto-von-Guericke University MagdeburgAnna Gos - University Hospital of ZurichKurt Trübner - University of Duisburg-EssenChristian Mawrin - Otto-von-Guericke University MagdeburgMichał Kaliszan - Gdańsk Medical UniversityThomas Nickl-Jockschat - Otto-von-Guericke University MagdeburgTomasz Gos - Otto-von-Guericke University Magdeburg
- Resource Type
- Journal article
- Publication Details
- European archives of psychiatry and clinical neuroscience, Vol.275(7), pp.2111-2118
- DOI
- 10.1007/s00406-025-01992-3
- PMID
- 40116918
- NLM abbreviation
- Eur Arch Psychiatry Clin Neurosci
- ISSN
- 1433-8491
- eISSN
- 1433-8491
- Publisher
- Springer Nature; HEIDELBERG
- Grant note
- BMBF NBL-3/2 / Bundesministerium für Bildung und Forschung N2-OVGU / Saxony-Anhalt Ministry of Research 07R-1832 / Stanley Medical Research Institute XN3594O/0405M / Saxony-Anhalt Ministry of Research 01ZZ0407 / Bundesministerium für Bildung und Forschung
- Language
- English
- Electronic publication date
- 03/21/2025
- Date published
- 10/2025
- Academic Unit
- Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984801840702771
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