Implant surface roughness affects osteoblast gene expression

G B Schneider; H Perinpanayagam; M Clegg; R Zaharias; D Seabold; J Keller; C Stanford

doi:10.1177/154405910308200509

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Implant surface roughness affects osteoblast gene expression

Journal article

Peer reviewed

Implant surface roughness affects osteoblast gene expression

G B Schneider, H Perinpanayagam, M Clegg, R Zaharias, D Seabold, J Keller and C Stanford

Journal of dental research, Vol.82(5), pp.372-376

05/2003

DOI: 10.1177/154405910308200509

PMID: 12709504

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Abstract

The transcription factor Cbfa1 regulates osteoblast differentiation and expression of genes necessary for the development of a mineralized phenotype. The purpose of this study was to determine if Cbfa1 and BSPII gene expression are influenced by implant surface microtopography. Osteoblasts were cultured on 600-grit (grooved) or sandblasted (roughened) cpTi implant discs. Mineralization was evaluated by Alizarin-Red-S staining. Real Time PCR was used for quantitative analysis of Cbfa1 and BSPII gene expression. Enhanced mineralization was seen in osteoblasts grown on roughened implant surfaces relative to tissue culture plastic. Real Time PCR showed significant (P < 0.05) increases in Cbfa1 gene expression in cells grown on roughened, as compared with grooved, implant surfaces. BSPII gene expression was also increased on rough surfaces in the UMR cells, but was reduced in the rat calvarial osteoblast cultures. These results suggest that osteoblast gene expression and mineralization are affected by roughened implant surface microtopographies during osseointegration of dental implants.

Gene Expression

Microscopy, Electron, Scanning

Integrin-Binding Sialoprotein

Cells, Cultured

Osseointegration - physiology

Rats

Transcription Factors - biosynthesis

Sialoglycoproteins - biosynthesis

Animals

Analysis of Variance

Calcification, Physiologic

Neoplasm Proteins

Surface Properties

Statistics, Nonparametric

Titanium

Cell Differentiation

Core Binding Factor Alpha 1 Subunit

Dental Implants

Osteoblasts - metabolism

Details

Title: Subtitle: Implant surface roughness affects osteoblast gene expression
Creators: G B Schneider - N402, Dows Institute for Dental Research and the Department of Prosthodontics, University of Iowa College of Dentistry, Iowa City 52242, USA. galen-schneider@uiowa.edu
H Perinpanayagam
M Clegg
R Zaharias
D Seabold
J Keller
C Stanford
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.82(5), pp.372-376
DOI: 10.1177/154405910308200509
PMID: 12709504
NLM abbreviation: J Dent Res
ISSN: 0022-0345
eISSN: 1544-0591
Publisher: United States
Grant note: P3010126-09 / PHS HHS P60DE13076 / NIDCR NIH HHS
Language: English
Date published: 05/2003
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Graduate College Admin and Gen; President; Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984066093802771

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