Journal article
Implications for in vitro studies of the autoxidation of ferrous ion and the iron-catalyzed autoxidation of dithiothreitol
Biochimica et biophysica acta. General subjects, Vol.719(3), pp.501-508
1982
DOI: 10.1016/0304-4165(82)90239-2
PMID: 7150657
Abstract
The influences of buffers and iron chelators on the rate of autoxidation of Fe
2+ were examined in the pH range 6.0–7.4. The catalysis by Fe
2+ and Fe
3+ of the autoxidation of dithiothreitol was also investigated. In buffers which are non- or poor chelators of iron, 0.25 mM Fe
2+, and 0.3 mM dithiothreitol when present with iron, oxidize within minutes at pH 7.4 and 30°C. The stability of each increases as the pH is decreased and more than 90% of each remains after 1 h at pH 6.0. In the presence of buffers or oxy-ligands which preferentially and strongly chelate Fe
3+ over Fe
2+, Fe
2+ autoxidizes rapidly in the pH range 6.0–7.4 while dithiothreitol is protected. Ligands which preferentially bind strongly to Fe
2+ stabilize both Fe
2+ and dithiothreitol at pH 7.4. Dithiothreitol readily reduces Fe
3+ in non-chelating buffers or in the presence of strong chelators of Fe
2+, however, the ferrous ions produced are prone to reoxidation at higher pH values. These results show that Fe
2+ and dithiothreitol are very susceptible to autoxidation in the neutral pH range, and that the rates are strongly influenced by the presence of chelators of Fe
2+ and Fe
3+. The rapid autoxidations of these species need to be taken into account when designing and interpreting experiments involving Fe
2+ or both dithiothreitol and iron.
Details
- Title: Subtitle
- Implications for in vitro studies of the autoxidation of ferrous ion and the iron-catalyzed autoxidation of dithiothreitol
- Creators
- David O. Lambeth - University of North DakotaGwen R. Ericson - University of North DakotaMark A. Yorek - University of North DakotaPaul D. Ray - University of North Dakota
- Resource Type
- Journal article
- Publication Details
- Biochimica et biophysica acta. General subjects, Vol.719(3), pp.501-508
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0304-4165(82)90239-2
- PMID
- 7150657
- ISSN
- 0304-4165
- eISSN
- 1872-8006
- Language
- English
- Date published
- 1982
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359766302771
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