Journal article
Importin-9 regulates chromosome segregation and packaging in Drosophila germ cells
Journal of cell science, Vol.134(7), jcs258391
04/01/2021
DOI: 10.1242/jcs.258391
PMCID: PMC8077489
PMID: 33632744
Abstract
Germ cells undergo distinct nuclear processes as they differentiate into gametes. Although these events must be coordinated to ensure proper maturation, the stage-specific transport of proteins in and out of germ cell nuclei remains incompletely understood. Our efforts to genetically characterize Drosophila genes that exhibit enriched expression in germ cells led to the finding that loss of the highly conserved Importin beta/karyopherin family member Importin-9 (Ipo9, herein referring to Ranbp9) results in female and male sterility. Immunofluorescence and fluorescent in situ hybridization revealed that Ipo9(KO) mutants display chromosome condensation and segregation defects during meiosis. In addition, Ipo9(KO) mutant males form abnormally structured sperm and fail to properly exchange histones for protamines. Ipo9 physically interacts with proteasome proteins, and Ipo9 mutant males exhibit disruption of the nuclear localization of several proteasome components. Thus, Ipo9 coordinates the nuclear import of functionally related factors necessary for the completion of gametogenesis.
This article has an associated First Person interview with the first author of the paper.
Details
- Title: Subtitle
- Importin-9 regulates chromosome segregation and packaging in Drosophila germ cells
- Creators
- Victor Palacios - The University of Texas Southwestern Medical CenterGarrett C. Kimble - Roy J. and Lucille A. Carver College of MedicineTina L. Tootle - Roy J. and Lucille A. Carver College of MedicineMichael Buszczak - The University of Texas Southwestern Medical Center
- Resource Type
- Journal article
- Publication Details
- Journal of cell science, Vol.134(7), jcs258391
- Publisher
- Company Biologists Ltd
- DOI
- 10.1242/jcs.258391
- PMID
- 33632744
- PMCID
- PMC8077489
- ISSN
- 0021-9533
- eISSN
- 1477-9137
- Number of pages
- 13
- Grant note
- R01GM116885; R01GM125812; R01AG047318 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 04/01/2021
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284329502771
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