Journal article
Improved Performance of a Rapid Immunochromatographic Assay for Detection of PBP2a in Non-Staphylococcus aureus Staphylococcal Species
Journal of clinical microbiology, Vol.57(4), e01417-18
04/2019
DOI: 10.1128/JCM.01417-18
PMCID: PMC6440771
PMID: 30651387
Abstract
Non-
staphylococcal species (non-SASS) are important pathogens in both animal and human populations. The development of β-lactam resistance in non-SASS through acquisition and expression of penicillin-binding protein 2a (PBP2a) represents a significant clinical and public health threat. Here, we evaluated the diagnostic performance of two versions of a PBP2a immunochromatographic assay with non-SASS. Our data show that the revised version of the assay, the PBP2a SA culture colony test, has superior diagnostic sensitivity compared to the previous version of the assay, the PBP2a culture colony test, 100% (95% confidence interval [CI], 93.3 to 100%) versus 67.9% (95% CI, 53.7 to 80.1%), respectively, while both assays display a specificity of 100% (95% CI, 92.5 to 100%). Therefore, the PBP2a SA culture colony test offers a rapid, accurate, and relatively inexpensive method for detecting PBP2a-mediated β-lactam resistance in clinically relevant non-SASS for the management of infections due to these organisms and for antimicrobial stewardship.
Details
- Title: Subtitle
- Improved Performance of a Rapid Immunochromatographic Assay for Detection of PBP2a in Non-Staphylococcus aureus Staphylococcal Species
- Creators
- Matthew C Canver - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USAMark D Gonzalez - Children's Healthcare of Atlanta, Atlanta, Georgia, USABradley A Ford - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, Iowa, USAAmanda R Arnold - Children's Healthcare of Atlanta, Atlanta, Georgia, USASara D Lawhon - Texas A&M University College of Veterinary Medicine, College Station, Texas, USAC A Burnham - Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, Missouri, USAStephen G Jenkins - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USAEileen M Burd - Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USALars F Westblade - Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York, USA law9067@med.cornell.edu
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.57(4), e01417-18
- Publisher
- United States
- DOI
- 10.1128/JCM.01417-18
- PMID
- 30651387
- PMCID
- PMC6440771
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Language
- English
- Date published
- 04/2019
- Academic Unit
- Pathology
- Record Identifier
- 9984046916502771
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