Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale

Fengzheng Gao; Marta Sá; Iago Teles Dominguez Cabanelas; René H. Wijffels; Maria J. Barbosa

doi:10.1016/j.biortech.2021.124725

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Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale

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Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale

Fengzheng Gao, Marta Sá, Iago Teles Dominguez Cabanelas, René H. Wijffels and Maria J. Barbosa

Bioresource technology, Vol.325, pp.124725-124725

04/01/2021

DOI: 10.1016/j.biortech.2021.124725

PMID: 33508680

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Abstract

[Display omitted] •Two rounds of directed evolution resulted in a novel Tisochrysis lutea phenotype.•Stable phenotype after 15 months and contamination resistance in outdoor production.•Fucoxanthin outdoor productivity was 3.1× higher than the original strain.•Docosahexaenoic acid (DHA) outdoor productivity was 1.6× higher than original strain.•Highest ever reported outdoor biomass, fucoxanthin and DHA productivities. This work aimed to select a Tisochrysis lutea phenotype with higher biomass and fucoxanthin productivities using fluorescence-activated cell sorting (FACS). A novel phenotype was obtained after 2 rounds of selection, based on high-fucoxanthin fluorescence. The resulting phenotype forms cell aggregates, has no flagella, and was stable after 15 months. Optimal temperature (30 °C) and light (300 µmol m−2 s−1) were obtained at laboratory scale, identical to the original strain. The biomass productivity was higher than the original strain: 1.9× at laboratory scale (0.4 L), and 4.5× under outdoor conditions (190 L). Moreover, compared to the original strain, the productivity of fucoxanthin increased 1.6–3.1× and docosahexaenoic acid 1.5–1.9×. These are the highest ever reported outdoor productivities, obtained with a robust new phenotype from a T. lutea monoculture isolated with FACS without genetic manipulation. The resulting phenotype shows high potential for industrial production.

Fluorescence-activated cell sorting (FACS)

Fucoxanthin

High productivity

Robust new phenotype

Tisochrysis lutea

Details

Title: Subtitle: Improved fucoxanthin and docosahexaenoic acid productivities of a sorted self-settling Tisochrysis lutea phenotype at pilot scale
Creators: Fengzheng Gao - Wageningen University & Research
Marta Sá - Wageningen University & Research
Iago Teles Dominguez Cabanelas - Wageningen University & Research
René H. Wijffels - Nord University
Maria J. Barbosa - Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700 AA Wageningen, Netherlands
Resource Type: Journal article
Publication Details: Bioresource technology, Vol.325, pp.124725-124725
DOI: 10.1016/j.biortech.2021.124725
PMID: 33508680
NLM abbreviation: Bioresour Technol
ISSN: 0960-8524
eISSN: 1873-2976
Publisher: Elsevier Ltd
Grant note: DOI: 10.13039/501100007601, name: Horizon 2020
Language: English
Date published: 04/01/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397914802771

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