Improved magnetic delivery of cells to the trabecular meshwork in mice

M Reza Bahrani Fard; Jessica Chan; Gabriela Sanchez Rodriguez; Marybeth Yonk; Shreya R Kuturu; A Thomas Read; Stanislav Y Emelianov; Markus H Kuehn; C Ross Ethier

doi:10.1016/j.exer.2023.109602

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Improved magnetic delivery of cells to the trabecular meshwork in mice

Journal article

Peer reviewed

Improved magnetic delivery of cells to the trabecular meshwork in mice

M Reza Bahrani Fard, Jessica Chan, Gabriela Sanchez Rodriguez, Marybeth Yonk, Shreya R Kuturu, A Thomas Read, Stanislav Y Emelianov, Markus H Kuehn and C Ross Ethier

Experimental eye research, Vol.234, 109602

09/2023

DOI: 10.1016/j.exer.2023.109602

PMCID: PMC10530071

PMID: 37488007

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https://pmc.ncbi.nlm.nih.gov/articles/PMC10530071/pdf/nihms-1921456.pdfView

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Abstract

Glaucoma is the leading cause of irreversible blindness worldwide and its most prevalent subtype is primary open angle glaucoma (POAG). One pathological change in POAG is loss of cells in the trabecular meshwork (TM), which is thought to contribute to ocular hypertension and has thus motivated development of cell-based therapies to refunctionalize the TM. TM cell therapy has shown promise in intraocular pressure (IOP) control, but existing cell delivery techniques suffer from poor delivery efficiency. We employed a novel magnetic delivery technique to reduce the unwanted side effects of off-target cell delivery. Mesenchymal stem cells (MSCs) were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) and after intracameral injection were magnetically steered towards the TM using a focused magnetic apparatus (“point magnet”). This technique delivered the cells significantly closer to the TM at higher quantities and with more circumferential uniformity compared to either unlabeled cells or those delivered using a “ring magnet” technique. We conclude that our point magnet cell delivery technique can improve the efficiency of TM cell therapy and in doing so, potentially increase the therapeutic benefits and lower the risk of complications such as tumorigenicity and immunogenicity.

Details

Title: Subtitle: Improved magnetic delivery of cells to the trabecular meshwork in mice
Creators: M Reza Bahrani Fard
Jessica Chan
Gabriela Sanchez Rodriguez
Marybeth Yonk
Shreya R Kuturu
A Thomas Read
Stanislav Y Emelianov
Markus H Kuehn
C Ross Ethier
Resource Type: Journal article
Publication Details: Experimental eye research, Vol.234, 109602
DOI: 10.1016/j.exer.2023.109602
PMID: 37488007
PMCID: PMC10530071
NLM abbreviation: Exp Eye Res
ISSN: 0014-4835
eISSN: 1096-0007
Grant note: DOI: 10.13039/100000053, name: National Eye Institute, award: R01 EY030071; DOI: 10.13039/100008065, name: Georgia Research Alliance; DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/501100005617, name: BHF Centre of Research Excellence, Oxford
Language: English
Date published: 09/2023
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9984448056102771

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