Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Ameneh Khatami; Tara M Randis; Anna Chamby; Thomas A Hooven; Margaret Gegick; Evan Suzman; Brady A'Hearn-Thomas; Andrew P Steenhoff; Adam J Ratner

doi:10.1093/ofid/ofy164

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Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Journal article

Open access

Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Ameneh Khatami, Tara M Randis, Anna Chamby, Thomas A Hooven, Margaret Gegick, Evan Suzman, Brady A'Hearn-Thomas, Andrew P Steenhoff and Adam J Ratner

Open Forum Infectious Diseases, Vol.5(7), pp.164-164

07/01/2018

DOI: 10.1093/ofid/ofy164

PMCID: PMC6051451

PMID: 30038931

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https://doi.org/10.1093/ofid/ofy164View

Published (Version of record)Open Forum Infectious Diseases, Volume 5, Issue 7, July 2018, ofy164

Abstract

Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

Medical Genetics

Pathology

Pediatrics

Pregnancy

Infectious Disease

Obstetrics and Gynecology

Streptococcus

GBS

PCR

sip

Group B streptococcus

polymerase chain reaction

Details

Title: Subtitle: Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides
Creators: Ameneh Khatami
Tara M Randis
Anna Chamby
Thomas A Hooven
Margaret Gegick
Evan Suzman
Brady A'Hearn-Thomas - University of Iowa
Andrew P Steenhoff
Adam J Ratner
Resource Type: Journal article
Publication Details: Open Forum Infectious Diseases, Vol.5(7), pp.164-164
DOI: 10.1093/ofid/ofy164
PMID: 30038931
PMCID: PMC6051451
NLM abbreviation: Open Forum Infect Dis
ISSN: 2328-8957
Grant note: Grant support K23 HD065844/HD/NICHD NIH HHS/United States R21 AI127957/AI/NIAID NIH HHS/United States R33 AI098654/AI/NIAID NIH HHS/United States
Comment: This work was supported by the Doris Duke Charitable Foundation (DDCF CSDA 2009–039 to A.J.R.) and the National Institutes of Health (R33 AI098654 to A.J.R.; K23 HD065844 and R21 AI127957 to T.M.R.). M.G. and E.S. were supported by the Jack Cary Eichenbaum Neonatology Scholars Program at New York University School of Medicine.
Language: English
Date published: 07/01/2018
Academic Unit: Stead Family Department of Pediatrics; Carver College of Medicine; Neonatology
Record Identifier: 9983557323402771

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