Journal article
In Situ Hybridization of SP-A mRNA in Adult Human Conducting Airways
Pediatric pathology & molecular medicine, Vol.20(5), pp.349-366
09/01/2001
DOI: 10.1080/15513810109168620
PMID: 11552737
Abstract
In our study, surfactant protein (SP)-A was characterized in adult human trachea and bronchi. SP-A mRNA and protein were localized to serous cells in submucosal gland by in situ hybridization and immunohistochemistry, respectively. A 2.2 kb SP-A mRNA transcript was detected in tracheal tissues by Northern blot analysis. Primer extension analysis and gene-specific reverse transcriptase polymerase chain reaction (RT-PCR) revealed the predominance of SP-A2 mRNA. However, using nested PCR, we also detected low amounts of SP-A1 mRNA in the tracheal tissues. A approximately 35 kDa SP-A immunoreactive protein was detected in the tracheal tissues by immunoblot analysis and was shown to be modified by the addition of N-linked oligosaccharides. We conclude that submucosal glands in the conducting airways produce a novel SP-A protein with a molecular weight and post-translational modification similar to the SP-A produced in the distal lung. We speculate that this SP-A2 protein, like other serous secretions from airway submucosal glands, functions in local antimicrobial host defense mechanisms in the conducting airways.
Details
- Title: Subtitle
- In Situ Hybridization of SP-A mRNA in Adult Human Conducting Airways
- Creators
- Kavita R KhubchandaniKelli L Goss - University of Iowa, Stead Family Department of PediatricsJohn F Engelhardt - University of Iowa, Anatomy and Cell BiologyJeanne M Snyder - University of Iowa, Stead Family Department of Pediatrics
- Resource Type
- Journal article
- Publication Details
- Pediatric pathology & molecular medicine, Vol.20(5), pp.349-366
- DOI
- 10.1080/15513810109168620
- PMID
- 11552737
- NLM abbreviation
- Pediatr Pathol Mol Med
- ISSN
- 1522-7952
- eISSN
- 1523-4525
- Language
- English
- Date published
- 09/01/2001
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025563602771
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