Journal article
In Vitro and In Vivo Efficacy of New Composite for Direct Pulp Capping
BioMed research international, Vol.2021, pp.8414577-10
2021
DOI: 10.1155/2021/8414577
PMCID: PMC8639243
PMID: 34869772
Abstract
To investigate physicochemical properties, dentin bonding, cytotoxicity, and in vivo pulp response of experimental self-adhesive composites tailored to direct pulp capping.
Experimental composites were prepared with beta-tricalcium phosphate and hydroxyapatite nanoparticles adsorbed with simvastatin and glutathione added at 0% (control resin), 1 wt% (Res 1%), and 10 wt% (Res 10%). A commercial light-curable calcium hydroxide (Ca(OH)
) (Ultra-Blend Plus) was used as control material. The physicochemical properties investigated were flexural strength and modulus, calcium release, and degree of conversion. Dentin bonding was assessed by the push-out test. Proliferation and cell counting assays were performed to evaluate in vitro cytotoxicity using fluorescence microscopy. In vivo pulp capping was performed on molars of Wistar rats, which were euthanized after 14 days and evaluated by histological analysis.
No statistical difference was observed in flexural strength and cell viability (
> 0.05). Res 10% presented higher modulus than control resin and Ca(OH)
. Also, Res 10% attained statistically higher degree of conversion when compared to other experimental composites. Ca(OH)
showed higher calcium release after 28 and 45 days of storage, with no statistical difference at 45 days to Res 10%. All experimental composites achieved significantly higher bond strength when compared to Ca(OH)
. While no significant difference was observed in the cell proliferation rates, resins at lower concentrations showed higher cell viability. In vivo evaluation of pulp response demonstrated no pulp damage with experimental composites.
The experimental composite investigated in this study achieved adequate physicochemical properties with minor in vivo pulpal inflammation and proved to be a valuable alternative for direct pulp capping.
Details
- Title: Subtitle
- In Vitro and In Vivo Efficacy of New Composite for Direct Pulp Capping
- Creators
- Julianne Coelho Silva - Postgraduate Program in Dentistry, Federal University of Ceará, Fortaleza, BrazilTainah Oliveira Rifane - Paulo Picanço School of DentistryAntônio Ernando Ferreira-Junior - Universidade Federal do CearáAna Paula Alves - Universidade Federal do CearáRichard Miron - University of BernYufeng Zhang - Wuhan UniversityPierre Basílio Almeida Fechine - Universidade Federal do CearáElayne Valério Carvalho - Universidade Federal do CearáVictor Pinheiro Feitosa - Paulo Picanço School of Dentistry
- Resource Type
- Journal article
- Publication Details
- BioMed research international, Vol.2021, pp.8414577-10
- DOI
- 10.1155/2021/8414577
- PMID
- 34869772
- PMCID
- PMC8639243
- NLM abbreviation
- Biomed Res Int
- ISSN
- 2314-6133
- eISSN
- 2314-6141
- Language
- English
- Date published
- 2021
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984654468502771
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