In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting

Paige T Richards; Jose Alberto Aguilar Briseño; Bethany A Brunton; Wendy Maury

doi:10.1007/978-1-0716-4256-6_13

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Journal article

In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting

Paige T Richards, Jose Alberto Aguilar Briseño, Bethany A Brunton and Wendy Maury

Methods in molecular biology (Clifton, N.J.), Vol.2877, pp.183-198

2025

DOI: 10.1007/978-1-0716-4256-6_13

PMCID: PMC11727417

PMID: 39585622

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https://pmc.ncbi.nlm.nih.gov/articles/PMC11727417/pdf/nihms-2043982.pdfView

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Abstract

Highly pathogenic viruses in the Filoviridae family are causative agents of filovirus disease (FVD). Ebola virus (EBOV) is one such member and, of all filoviruses, represents the largest threat to global public health. The study of FVD has been hampered by the lack of tools to study filovirus infection outside maximum containment laboratories. Recombinant vesicular stomatitis virus (VSV) lacking its native glycoprotein and expressing a filovirus glycoprotein (VSV-filo GP) has improved our understanding of GP-mediated host-cell interactions as well as adaptive and humoral immune responses in in vitro and in vivo studies. Furthermore, mouse models suitable for these studies are readily available. Here, we describe multiple injection routes for investigating filovirus GP-mediated infection and pathogenesis using VSV-filo GP and interferon α/β receptor-deficient (Ifnar ) mice as models. These tools can be safely used outside maximum containment laboratories, are cost effective, and easy to manipulate.

Animals

Containment of Biohazards - methods

Disease Models, Animal

Ebolavirus - genetics

Ebolavirus - pathogenicity

Filoviridae - genetics

Filoviridae - pathogenicity

Filoviridae Infections - virology

Glycoproteins - genetics

Glycoproteins - metabolism

Humans

Mice

Mice, Knockout

Receptor, Interferon alpha-beta - deficiency

Receptor, Interferon alpha-beta - genetics

Receptor, Interferon alpha-beta - metabolism

Vesiculovirus - genetics

Vesiculovirus - physiology

Viral Envelope Proteins - genetics

Viral Envelope Proteins - metabolism

Details

Title: Subtitle: In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting
Creators: Paige T Richards - University of Iowa
Jose Alberto Aguilar Briseño - University of Iowa, Microbiology and Immunology
Bethany A Brunton - University of Iowa
Wendy Maury - University of Iowa
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.2877, pp.183-198
DOI: 10.1007/978-1-0716-4256-6_13
PMID: 39585622
PMCID: PMC11727417
NLM abbreviation: Methods Mol Biol
eISBN: 9781071642566; 1071642561
ISSN: 1064-3745
eISSN: 1940-6029
Language: English
Date published: 2025
Academic Unit: Microbiology and Immunology
Record Identifier: 9984752658302771

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