Journal article
In Vivo Replication and Reversion to Wild Type of a Neutralization-Resistant Antigenic Variant of Hepatitis A Virus
The Journal of infectious diseases, Vol.161(1), pp.7-13
01/1990
DOI: 10.1093/infdis/161.1.7
PMID: 1688601
Abstract
Six seronegative owl monkeys were intravenously inoculated with an antigenic variant (SI8) of hepatitis A virus that is highly adapted to growth in cell culture and resists neutralization by monoclonal antibodies due to replacement of aspartic acid 70 of capsid protein VP3 with histidine. Each developed hepatitis 22–33 days after inoculation. Virus in feces, serum, and liver was quantified by radioimmunofocus assay. Viremia developed 7–11 days after inoculation, in parallel with fecal shedding of virus, and persisted for a mean of 20.5 days. Although the antigenic variant was recovered from feces or liver of three animals, virus in liver at the time of enzyme elevations was predominantly wild-type antigenic phenotype. Virus was not recoveredfrom liver 96 days after challenge. These studies further define virologic events in hepatitis A and show that in vivo replication of an antigenic variant was restricted compared with that of wildtype virus.
Details
- Title: Subtitle
- In Vivo Replication and Reversion to Wild Type of a Neutralization-Resistant Antigenic Variant of Hepatitis A Virus
- Creators
- Stanley M Lemon - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandLeonard N Binn - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandRuth Marchwicki - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandPaula C Murphy - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandLi-Hua Ping - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandRobert w Jansen - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandLudmilla V. S Asher - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandJack T Stapleton - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandDewayne G Taylor - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, MarylandJames W LeDuc - Division of Infectious Diseases, Department of Medicine, University of North Carolina, Chapel Hill, Washington, DC, Fort Detrick, Frederick, Maryland
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.161(1), pp.7-13
- Publisher
- The University of Chicago Press
- DOI
- 10.1093/infdis/161.1.7
- PMID
- 1688601
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 01/1990
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094516002771
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