In experimental peripheral arterial disease, type 2 diabetes alters post-ischemic gene expression

Rahul Peravali; Lucas Gunnels; Karthik Dhanabalan; Folabi Ariganjoye; Ivan C. Gerling; Ayotunde O. Dokun

doi:10.1016/j.jcte.2019.100199

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In experimental peripheral arterial disease, type 2 diabetes alters post-ischemic gene expression

Journal article

Open access

Peer reviewed

In experimental peripheral arterial disease, type 2 diabetes alters post-ischemic gene expression

Rahul Peravali, Lucas Gunnels, Karthik Dhanabalan, Folabi Ariganjoye, Ivan C. Gerling and Ayotunde O. Dokun

Journal of clinical & translational endocrinology, Vol.17, pp.100199-100199

09/2019

DOI: 10.1016/j.jcte.2019.100199

PMCID: PMC6595134

PMID: 31293900

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Abstract

Peripheral arterial disease is characterized by impaired blood flow to tissues outside the heart due to atherosclerosis and it most frequently occurs in the lower extremities. Type 2 diabetes (T2D) is a well-known risk factor that accelerate the course and contributes to poor clinical outcomes of PAD. While there is some evidence that T2D is associated with altered expression of genes involved in regulating PAD severity, our knowledge about the specific genes and pathways involved remains incomplete. We induced experimental PAD or hind limb ischemia in T2D and non-diabetic mice and subjected the ischemic gastrocnemius muscle tissues to genome-wide mRNA transcriptome analysis. We subsequently performed pathway analysis on the top 500 genes that showed the most significant expression differences between the ischemic diabetic and ischemic non-diabetic muscle tissues. Pathway analysis of the differentially expressed genes identified pathways involved in essential biological processes such as “metabolic pathways,” “phagosomes,” “lysosomes,” and “regulation of actin cytoskeleton”. Overall, our data provides the opportunity to test hypotheses on the potential role of the altered genes/molecular pathways in poor PAD outcomes in diabetes.

Genome-wide gene expression profiling

Molecular pathway analysis

Peripheral artery disease

Type 2 diabetes mellitus

Details

Title: Subtitle: In experimental peripheral arterial disease, type 2 diabetes alters post-ischemic gene expression
Creators: Rahul Peravali - University of Tennessee Health Science Center
Lucas Gunnels - University of Tennessee Health Science Center
Karthik Dhanabalan - University of Tennessee Health Science Center
Folabi Ariganjoye - University of Tennessee Health Science Center
Ivan C. Gerling - University of Tennessee Health Science Center
Ayotunde O. Dokun - University of Tennessee Health Science Center
Resource Type: Journal article
Publication Details: Journal of clinical & translational endocrinology, Vol.17, pp.100199-100199
DOI: 10.1016/j.jcte.2019.100199
PMID: 31293900
PMCID: PMC6595134
NLM abbreviation: J Clin Transl Endocrinol
ISSN: 2214-6237
eISSN: 2214-6237
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000050, name: National Heart, Lung, and Blood Institute, award: R01 HL130399; name: Short-Term Research Training, award: T32-5T35DK113964-02; DOI: 10.13039/100000062, name: National Institute of Diabetes and Digestive and Kidney Diseases
Language: English
Date published: 09/2019
Academic Unit: Molecular Physiology and Biophysics; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984297601502771

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