Journal article
In situ single photon confocal imaging of cardiomyocyte T-tubule system from Langendorff-perfused hearts
Frontiers in physiology, Vol.6(MAY), pp.134-134
05/06/2015
DOI: 10.3389/fphys.2015.00134
PMCID: PMC4422017
PMID: 25999861
Abstract
Transverse tubules (T-tubules) are orderly invaginations of the sarcolemma in mammalian cardiomyocytes. The integrity of T-tubule architecture is critical for cardiac excitation contraction coupling function. T-tubule remodeling is recognized as a key player in cardiac dysfunction. Early studies on T-tubule structure were based on electron microscopy, which uncovered important information about the T-tubule architecture. The advent of fluorescent membrane probes allowed the application of confocal microscopy to investigations of T-tubule structure. Studies have now been extended beyond single cardiomyocytes to examine the T-tubule network in intact hearts through in situ confocal imaging of Langendorff-perfused hearts. This technique has allowed visualization of T-tubule organization in their natural habitat, avoiding the damage induced by isolation of cardiomyocytes. Additionally, it is possible to obtain T-tubule images in different subepicardial regions in a single intact heart. We review how this state-of-the-art imaging technique has provided important mechanistic insights into maturation of T-tubules in developing hearts and defined the role of T-tubule remodeling in development and progression of heart failure.
Details
- Title: Subtitle
- In situ single photon confocal imaging of cardiomyocyte T-tubule system from Langendorff-perfused hearts
- Creators
- Biyi Chen - Roy J. and Lucille A. Carver College of MedicineCaimei Zhang - Roy J. and Lucille A. Carver College of MedicineAng Guo - Roy J. and Lucille A. Carver College of MedicineLong-Sheng Song - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Frontiers in physiology, Vol.6(MAY), pp.134-134
- DOI
- 10.3389/fphys.2015.00134
- PMID
- 25999861
- PMCID
- PMC4422017
- NLM abbreviation
- Front Physiol
- ISSN
- 1664-042X
- eISSN
- 1664-042X
- Publisher
- Frontiers Media Sa
- Number of pages
- 9
- Grant note
- R01 HL090905 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 13POST14630077 / American Heart Association Midwest Postdoctoral Fellowship; American Heart Association R01HL090905 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) 0635056N / American Heart Association Scientific Development Grant; American Heart Association
- Language
- English
- Date published
- 05/06/2015
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984293082702771
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