Journal article
In vitro and in vivo comparisons of staphylococcal biofilm formation on a cross-linked poly(ethylene glycol)-based polymer coating
Acta biomaterialia, Vol.6(3), pp.1119-1124
03/01/2010
DOI: 10.1016/j.actbio.2009.08.040
PMID: 19733265
Abstract
Poly(ethylene glycol) (PEG) coatings are known to reduce microbial adhesion in terms of numbers and binding strength. However, bacterial adhesion remains of the order of 10
4
cm
−2. It is unknown whether this density of bacteria will eventually grow into a biofilm. This study investigates the kinetics of staphylococcal biofilm formation on a commercially produced, robust, cross-linked PEG-based polymer coating (OptiChem
®) in vitro and in vivo. OptiChem
® inhibits biofilm formation in vitro, and although adsorption of plasma proteins encourages biofilm formation, microbial growth kinetics are still strongly delayed compared to uncoated glass. In vivo, OptiChem
®-coated and bare silicone rubber samples were inserted into an infected murine subcutaneous pocket model. In contrast to bare silicone rubber, OptiChem
® samples did not become colonized upon reimplantation despite the fact that surrounding tissues were always culture-positive. We conclude that the commercial OptiChem
® coating considerably slows down bacterial biofilm formation both in vitro and in vivo, making it an attractive candidate for biomaterials implant coating.
Details
- Title: Subtitle
- In vitro and in vivo comparisons of staphylococcal biofilm formation on a cross-linked poly(ethylene glycol)-based polymer coating
- Creators
- Isabel C. Saldarriaga Fernández - University Medical Center GroningenHenny C Van der Mei - University Medical Center GroningenSteve Metzger - ARC TechnologyDavid W. Grainger - University of UtahAnton F. Engelsman - University Medical Center GroningenM. Reza Nejadnik - University Medical Center GroningenHenk J. Busscher - University Medical Center Groningen
- Resource Type
- Journal article
- Publication Details
- Acta biomaterialia, Vol.6(3), pp.1119-1124
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.actbio.2009.08.040
- PMID
- 19733265
- ISSN
- 1742-7061
- eISSN
- 1878-7568
- Language
- English
- Date published
- 03/01/2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420836602771
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