In vitro antibacterial efficacy of endodontic irrigants against Enterococcus faecalis

Daniel P Oliveira; Joao V B Barbizam; Martin Trope; Fabricio B Teixeira

doi:10.1016/j.tripleo.2006.11.007

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Journal article

In vitro antibacterial efficacy of endodontic irrigants against Enterococcus faecalis

Daniel P Oliveira, Joao V B Barbizam, Martin Trope and Fabricio B Teixeira

Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, Vol.103(5), pp.702-706

05/2007

DOI: 10.1016/j.tripleo.2006.11.007

PMID: 17368057

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Abstract

The objective of this study was to compare the in vitro antimicrobial activity of 2% chlorhexidine gel against Enterococcus faecalis with sodium hypochlorite in 2 different concentrations (1.5% and 5.25%). Eighty human lower premolars with single root canals were prepared, autoclaved, and infected for 7 days with E. faecalis monocultures. The roots were then separated into 5 experimental groups according to the irrigant solution used during the standardized preparation. To assess the antimicrobial action of the irrigant solutions, 3 microbial samples were taken: S1--initial (before the biomechanical preparation), S2--posttreatment (immediately after the biomechanical preparation), and S3--final (7 days after the biomechanical preparation). The microbiological samples were plated to count the colony-forming units (CFU). The 2% chlorhexidine gel and 5.25% sodium hypochlorite significantly reduced the E. faecalis CFU in the posttreatment and final microbiological samples. The 1.5% sodium hypochlorite also reduced the E. faecalis CFU immediately after the root canal instrumentation, but the E. faecalis CFU increased in the final sample showing no statistical difference from the control group. The 2% chlorhexidine gluconate gel and 5.25% sodium hypochlorite were effective in eliminating E. faecalis even 7 days after the instrumentation; moreover, the higher the concentration of sodium hypochlorite the better its antimicrobial action.

Enterococcus faecalis - drug effects

Anti-Infective Agents, Local - pharmacology

Humans

Chlorhexidine - pharmacology

Root Canal Irrigants - administration & dosage

Colony Count, Microbial

Sodium Hypochlorite - administration & dosage

Dental Pulp Cavity - microbiology

Root Canal Irrigants - pharmacology

Dose-Response Relationship, Drug

Anti-Infective Agents, Local - administration & dosage

Statistics, Nonparametric

Chlorhexidine - administration & dosage

Sodium Hypochlorite - pharmacology

Details

Title: Subtitle: In vitro antibacterial efficacy of endodontic irrigants against Enterococcus faecalis
Creators: Daniel P Oliveira - University of North Carolina School of Dentistry, Chapel Hill, NC, USA
Joao V B Barbizam
Martin Trope
Fabricio B Teixeira
Resource Type: Journal article
Publication Details: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, Vol.103(5), pp.702-706
Publisher: United States
DOI: 10.1016/j.tripleo.2006.11.007
PMID: 17368057
ISSN: 1079-2104
eISSN: 1528-395X
Language: English
Date published: 05/2007
Academic Unit: Endodontics
Record Identifier: 9984065702402771

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