Journal article
In-vitro enzymatic biodegradation of adhesive resin in the hybrid layer
Journal of biomedical materials research. Part A, Vol.94(1), pp.187-192
07/2010
DOI: 10.1002/jbm.a.32689
PMCID: PMC2915822
PMID: 20127999
Abstract
Penetration of adhesives into the demineralized dentin surface and their subsequent conversion are critically important to longevity of the adhesive resin-dentin bond. The durability of the resin-dentin bond is investigated by monitoring the change of adhesive concentration within the hybrid layer of aged specimens using Raman spectroscopy. Absolute molar concentrations of Bis-GMA and HEMA were measured across the hybrid layer of resin-dentin specimens 24 h after photopolymerization and after 24-wk storage in one of three media: artificial saliva (SAL), SAL containing cholesterol esterase to attack resin (EST), and SAL containing bacterial collagenase to attack collagen (COL). No significant difference among these groups for both Bis-GMA and HEMA molar concentrations at 24-h storage was found; however, concentrations decreased from the adhesive resin to the middle of the hybrid layer. Concentrations remained unchanged at any resin-dentin position after aging in SAL. In the hybrid layer, concentrations significantly decreased with aging in COL and tended to decrease in EST. While showing potential enzymatic biodegradative effects of endogenous matrix metalloproteinases and salivary esterases, this methodology may also prove to be a valuable assessment of new chemistries and future approaches to improve resin-dentin bond performance.
Details
- Title: Subtitle
- In-vitro enzymatic biodegradation of adhesive resin in the hybrid layer
- Creators
- Yuan Zou - Department of Chemical & Biochemical Engineering, University of Iowa, Iowa City, IA, 52242, USAJulie L. P Jessop - Department of Chemical & Biochemical Engineering, University of Iowa, Iowa City, IA, 52242, USASteven R Armstrong - Department of Operative Dentistry, University of Iowa, Iowa City, IA, 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of biomedical materials research. Part A, Vol.94(1), pp.187-192
- DOI
- 10.1002/jbm.a.32689
- PMID
- 20127999
- PMCID
- PMC2915822
- NLM abbreviation
- J Biomed Mater Res A
- ISSN
- 1549-3296
- eISSN
- 1552-4965
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Operative Dentistry; Chemical and Biochemical Engineering
- Record Identifier
- 9984065806102771
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