Journal article
In vitro evidence for role of ERK, p38, and JNK in exocrine pancreatic cytokine production
Journal of gastrointestinal surgery, Vol.10(10), pp.1376-1383
10/2006
DOI: 10.1016/j.gassur.2006.09.003
PMID: 17175457
Abstract
Elucidation of mechanisms of acinar cell cytokine production is essential for a better understanding of acute pancreatitis pathogenesis. We hypothesize that the stress kinases ERK, p38, and JNK play an important role in acinar cell cytokine production. Rat pancreatic fragments were incubated with 100 nM concentration of the cholecystokinin analog caerulein or 100 nM caerulein and specific ERK inhibitor (100 µM PD98059), specific p38 inhibitor (10 µM SB203580), or specific JNK inhibitor (20 µM SP600125). After 3 hours of caerulein treatment, pancreatic fragments were homogenized and assayed for total and phosphorylated ERK, p38, and JNK, and for tumor necrosis factor-α or interleukin-1β concentrations (ELISA). Pancreatic fragments stimulated with caerulein showed activation of ERK, p38, and JNK and increased cytokine concentrations (ANOVA, P<0.05). Specific stress kinase inhibitors significantly attenuated caerulein-induced activation of the corresponding stress kinase and cytokine production; however, the effect of the JNK inhibitor was comparatively less convincing. Increased activation of ERK, p38, and JNK in pancreatic fragments was not associated with significant increases in total ERK, total p38, or total JNK concentrations. The stress kinases ERK and p38 play an important role in caerulein-stimulated exocrine pancreatic overproduction of cytokines. The role of JNK needs further evaluation in this experimental model.
Details
- Title: Subtitle
- In vitro evidence for role of ERK, p38, and JNK in exocrine pancreatic cytokine production
- Creators
- Isaac Samuel - Veterans Affairs Medical Center Iowa City IAAsgar Zaheer - Veterans Affairs Medical Center Iowa City IARory Fisher - Department of Pharmacology University of Iowa Roy J. and Lucille A. Carver College of Medicine Iowa City Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of gastrointestinal surgery, Vol.10(10), pp.1376-1383
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- DOI
- 10.1016/j.gassur.2006.09.003
- PMID
- 17175457
- ISSN
- 1091-255X
- eISSN
- 1873-4626
- Language
- English
- Date published
- 10/2006
- Academic Unit
- Neurology; Iowa Neuroscience Institute; Surgery; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040337202771
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