In vitro human cell line models to predict clinical response to anticancer drugs

Nifang Niu; Liewei Wang

doi:10.2217/pgs.14.170

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In vitro human cell line models to predict clinical response to anticancer drugs

Journal article

Peer reviewed

In vitro human cell line models to predict clinical response to anticancer drugs

Nifang Niu and Liewei Wang

Pharmacogenomics, Vol.16(3), pp.273-285

03/01/2015

DOI: 10.2217/pgs.14.170

PMCID: PMC4358765

PMID: 25712190

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https://www.ncbi.nlm.nih.gov/pmc/articles/4358765View

Open Access

Abstract

In vitro human cell line models have been widely used for cancer pharmacogenomic studies to predict clinical response, to help generate pharmacogenomic hypothesis for further testing, and to help identify novel mechanisms associated with variation in drug response. Among cell line model systems, immortalized cell lines such as Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) have been used most often to test the effect of germline genetic variation on drug efficacy and toxicity. Another model, especially in cancer research, uses cancer cell lines such as the NCI-60 panel. These models have been used mainly to determine the effect of somatic alterations on response to anticancer therapy. Even though these cell line model systems are very useful for initial screening, results from integrated analyses of multiple omics data and drug response phenotypes using cell line model systems still need to be confirmed by functional validation and mechanistic studies, as well as validation studies using clinical samples. Future models might include the use of patient-specific inducible pluripotent stem cells and the incorporation of 3D culture which could further optimize in vitro cell line models to improve their predictive validity.

Antineoplastic Agents - therapeutic use

Breast Neoplasms - drug therapy

Breast Neoplasms - genetics

Cell Line, Transformed - drug effects

Cell Line, Transformed - virology

Cell Line, Tumor - drug effects

Colorectal Neoplasms - drug therapy

Colorectal Neoplasms - genetics

Drug Screening Assays, Antitumor - methods

Female

Genetic Markers

Genetic Variation

HapMap Project

Herpesvirus 4, Human

Humans

In Vitro Techniques

Induced Pluripotent Stem Cells - drug effects

Models, Biological

Polymorphism, Single Nucleotide

Tumor Microenvironment

Details

Title: Subtitle: In vitro human cell line models to predict clinical response to anticancer drugs
Creators: Nifang Niu - Mayo Clinic
Liewei Wang - Mayo Clinic
Resource Type: Journal article
Publication Details: Pharmacogenomics, Vol.16(3), pp.273-285
DOI: 10.2217/pgs.14.170
PMID: 25712190
PMCID: PMC4358765
NLM abbreviation: Pharmacogenomics
ISSN: 1462-2416
eISSN: 1744-8042
Grant note: U19 GM61388 / NIGMS NIH HHS U19 GM061388 / NIGMS NIH HHS R01 CA138461 / NCI NIH HHS
Language: English
Date published: 03/01/2015
Academic Unit: Stead Family Department of Pediatrics; Medical Genetics and Genomics
Record Identifier: 9984701655202771

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