Journal article
In vitro interaction of fluconazole and amphotericin B administered sequentially against Candida albicans: effect of concentration and exposure time
Diagnostic microbiology and infectious disease, Vol.32(3), pp.205-210
1998
DOI: 10.1016/S0732-8893(98)00099-6
PMID: 9884837
Abstract
This study investigated the potential antagonism of fluconazole on amphotericin B activity against
Candida albicans when administered sequentially in vitro. Yeast cells were exposed to fluconazole for time periods ranging from 0 to 24 h before the addition of amphotericin B. The combination showed fungicidal (≥3 log
10 reduction in CFU/mL) activity. After 4 h of exposure to fluconazole, amphotericin B activity was partially inhibited at the lower concentration tested (0.25 × MIC). Amphotericin B activity was dramatically decreased by previous exposure to fluconazole for greater than or equal to 8 h at both the high and low concentrations tested. The activity of amphotericin B against yeast exposed to fluconazole for at least 8 h was indistinguishable from fluconazole alone and was fungistatic (≤2 log
10 reduction in CFU/mL). This inhibition of amphotericin B activity persisted for a very short period (<6 h) after removal of fluconazole from the culture medium, indicating the need for continued exposure to fluconazole for lasting inhibition of amphotericin B activity.
Details
- Title: Subtitle
- In vitro interaction of fluconazole and amphotericin B administered sequentially against Candida albicans: effect of concentration and exposure time
- Creators
- Erika J. Ernst - University of IowaMichael E. Klepser - University of IowaMichael A. Pfaller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.32(3), pp.205-210
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0732-8893(98)00099-6
- PMID
- 9884837
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1998
- Academic Unit
- Epidemiology; Pathology; Pharmacy Practice and Science
- Record Identifier
- 9984366041402771
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