Journal article
In vitro pharmacodynamic properties of MK-0991 determined by time-kill methods
Diagnostic microbiology and infectious disease, Vol.33(2), pp.75-80
1999
DOI: 10.1016/S0732-8893(98)00130-8
PMID: 10091029
Abstract
MK-0991 has demonstrated activity against a variety of fungal pathogens. We evaluated the MIC endpoint for MK-0991 by reading the endpoint using three methods and comparing these results with minimum fungicidal concentrations and electron micrographs. The concentration that resulted in 80% inhibition of fungal growth compared with control, similar to the endpoint for the azole antifungal agents, provided the most consistent results. Additionally, we investigated the time-kill properties of this agent against two isolates each of
Candida albicans,
Candida glabrata and
Candida tropicalis at concentrations ranging from 0.125 × MIC to 16 × MIC. Kill curves were performed using RPMI buffered with morpholine propanesulfonic acid as growth media. Samples were obtained at predetermined time points over 24 h and plated for colony counting. Fungicidal activity was observed with one isolate of
C. albicans, two isolates of
C. glabrata, and one isolate of
C. tropicalis. MK-0991 displayed concentration-dependent activity, which was fungicidal or fungistatic depending on the isolate tested.
Details
- Title: Subtitle
- In vitro pharmacodynamic properties of MK-0991 determined by time-kill methods
- Creators
- Erika J Ernst - University of IowaMichael E Klepser - University of IowaMichael E Ernst - University of IowaShawn A Messer - University of IowaMichael A Pfaller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.33(2), pp.75-80
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0732-8893(98)00130-8
- PMID
- 10091029
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1999
- Academic Unit
- Epidemiology; Pathology; Family and Community Medicine; Pharmacy Practice and Science
- Record Identifier
- 9984297337602771
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