Journal article
In vitro spontaneous parthenogenetic activation of golden hamster oocytes
Theriogenology, Vol.57(2), pp.845-851
2002
DOI: 10.1016/S0093-691X(01)00680-X
PMID: 11991388
Abstract
Parthenogenetic activation is a major hurdle to be cleared for the examination of the human sperm chromosome after intracytoplasmic injection (ICSI) into golden hamster oocytes. Various factors that affect spontaneous activation of hamster oocytes were, therefore, investigated in this study. We collected cumulus–oocyte complexes (COC) from the oviducts of superovulated females and washed them thoroughly with Ca
2+-containing or Ca
2+-free TALP-HEPES medium (handling media). We cultured oocytes with intact cumulus or those without cumulus (removed by previous hyaluronidase treatment) in Ca
2+-containing or -free m-TALP-3 for 6 or 12
h before examining for their activation. Among the oocytes recovered 17
h post-hCG, 92–94% were parthenogenetically activated by 6
h of in vitro culture. Activation rate in the oocytes collected at 13.5
h post-hCG (53%) was significantly (
P<0.05) lower than that in the oocytes collected 17
h post-hCG (92%), indicating that the spontaneous activation rate increased as the oocytes became older. Both cumulus-intact and cumulus-free oocytes had similar (
P>0.05) activation rates when cultured in vitro, suggesting that hyaluronidase treatment had no effect on the rate of oocyte activation. Omission of Ca
2+ from the handling medium also had no effect on the activation of the oocytes. The rate of spontaneous activation of the oocytes cultured in calcium-free medium for 6 (9%) and 12
h (16%) was significantly (
P<0.01) lower than that (94%) of the control oocytes cultured in Ca
2+-containing medium, implying a positive influence of Ca
2+ on in vitro activation of hamster oocytes. When we cultured the oocytes first in calcium-free medium for 6
h, and then in calcium-containing medium for 6
h, 94% were activated, which is comparable to the rate for oocytes continuously cultured in Ca
2+-containing medium. This indicates that the inhibition of hamster oocyte activation in Ca
2+-free medium is reversible and can be used to control spontaneous activation of golden hamster oocytes.
Details
- Title: Subtitle
- In vitro spontaneous parthenogenetic activation of golden hamster oocytes
- Creators
- X.S. Sun - Northeast Agricultural UniversityK.Z. Yue - Northeast Agricultural UniversityJ.B. Zhou - Northeast Agricultural UniversityQ.X. Chen - The Institute of Developmental Biology, China Academy of Sciences, Beijing, 100080, PR ChinaJ.H. Tan - Shandong Agricultural University
- Resource Type
- Journal article
- Publication Details
- Theriogenology, Vol.57(2), pp.845-851
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0093-691X(01)00680-X
- PMID
- 11991388
- ISSN
- 0093-691X
- eISSN
- 1879-3231
- Language
- English
- Date published
- 2002
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284347502771
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