Journal article
In vitro study of the interaction of doxorubicin, thiotepa, and mitomycin-C, agents used for intravesical chemotherapy of superficial bladder cancer
The Journal of urology, Vol.145(3), pp.613-617
1991
DOI: 10.1016/S0022-5347(17)38406-9
PMID: 1900087
Abstract
Several cytotoxic agents have been identified as effective in the treatment of superficial transitional cell carcinoma of the bladder, including doxorubicin, thiotepa and mitomycin-C. An in vitro study was conducted to assess the interactions of these three drugs against a well differentiated human bladder tumor cell line, RT-4, to identify and evaluate synergistic combinations among these agents. Cytotoxicity was evaluated by a colorimetric assay based on the capacity of viable cells to metabolize a tetrazolium dye, MTT, to produce a colored formazan product. The analyses of drug interactions were done by the isobolographic method (construction of isoeffect plots). The combination of doxorubicin and thiotepa was found to be the most synergistic, followed by the combination of doxorubicin and mitomycin-C. The combination of mitomycin C and thiotepa demonstrated an unpredictable effect. These findings suggest the combination of doxorubicin and thiotepa has potential advantage for chemotherapy of superficial bladder tumors.
Details
- Title: Subtitle
- In vitro study of the interaction of doxorubicin, thiotepa, and mitomycin-C, agents used for intravesical chemotherapy of superficial bladder cancer
- Creators
- L. A Seraphim - Roswell Park Cancer InstituteS. D Perrapato - Roswell Park Cancer InstituteH. K Slocum - Roswell Park Cancer InstituteY. M Rustum - Roswell Park Cancer InstituteR. P Huben - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- The Journal of urology, Vol.145(3), pp.613-617
- Publisher
- Lippincott Williams & Wilkins
- DOI
- 10.1016/S0022-5347(17)38406-9
- PMID
- 1900087
- ISSN
- 0022-5347
- eISSN
- 1527-3792
- Language
- English
- Date published
- 1991
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359583002771
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