In vitro susceptibility testing of Aspergillus spp.: comparison of Etest and reference microdilution methods for determining voriconazole and itraconazole MICs

J B Pfaller; S A Messer; R J Hollis; D J Diekema; M A Pfaller

doi:10.1128/JCM.41.3.1126-1129.2003

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In vitro susceptibility testing of Aspergillus spp.: comparison of Etest and reference microdilution methods for determining voriconazole and itraconazole MICs

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In vitro susceptibility testing of Aspergillus spp.: comparison of Etest and reference microdilution methods for determining voriconazole and itraconazole MICs

J B Pfaller, S A Messer, R J Hollis, D J Diekema and M A Pfaller

Journal of clinical microbiology, Vol.41(3), pp.1126-1129

03/2003

DOI: 10.1128/JCM.41.3.1126-1129.2003

PMCID: PMC150323

PMID: 12624040

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Abstract

The performance of the Etest for voriconazole and for itraconazole susceptibility testing of 376 isolates of Aspergillus spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards (NCCLS) proposed standard microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48 h at 35 degrees C. Etest MICs were determined with RPMI agar containing 2% glucose and were read after incubation for 48 h at 35 degrees C. The isolates included A. fumigatus, A. flavus, A. niger, A. terreus, A. versicolor, A. glaucus, A. nidulans, A. ustus, and A. sydowii. Overall agreement percentages between the Etest and microdilution MICs were 97.6% for voriconazole and 95.8% for itraconazole. Where a discrepancy was observed between Etest and the reference method, the Etest tended to give lower values with voriconazole and higher values with itraconazole. The Etest method using RPMI agar appears to be a useful method for determining the voriconazole and itraconazole susceptibilities of Aspergillus spp.

Antifungal Agents - pharmacology

Triazoles - pharmacology

Humans

Voriconazole

Microbiological Techniques

Pyrimidines - pharmacology

Microbial Sensitivity Tests - methods

Itraconazole - pharmacology

Aspergillus - drug effects

Details

Title: Subtitle: In vitro susceptibility testing of Aspergillus spp.: comparison of Etest and reference microdilution methods for determining voriconazole and itraconazole MICs
Creators: J B Pfaller - Departments of Pathology. Medicine. Epidemiology, University of Iowa College of Medicine and College of Public Health, Iowa City, Iowa 52242, USA
S A Messer
R J Hollis
D J Diekema
M A Pfaller
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.41(3), pp.1126-1129
DOI: 10.1128/JCM.41.3.1126-1129.2003
PMID: 12624040
PMCID: PMC150323
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 03/2003
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986371002771

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