Journal article
In vitro susceptibility testing of filamentous fungi: comparison of Etest and reference M38-A microdilution methods for determining posaconazole MICs
Diagnostic microbiology and infectious disease, Vol.45(4), pp.241-244
2003
DOI: 10.1016/S0732-8893(02)00544-8
PMID: 12729993
Abstract
The performance of the Etest for posaconazole susceptibility testing of 72 isolates of filamentous fungi was assessed in comparison with the National Committee for Clinical Laboratory Standards (NCCLS) approved standard broth microdilution method (M38-A). The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48h at 35°C. Etest MICs were determined with RPMI agar containing 2% glucose and were read after incubation for 48h at 35°C. The isolates included
Aspergillus fumigatus, A. flavus, A. nidulans, A. niger, A. versicolor, A. oryzae, A. terreus, Cladosporium spp.,
Curvularia sp.,
Exophiala sp.,
Fusarium spp.,
Paecilomyces spp.,
Pithomyces sp.,
Penicillium spp. and
Scedosporium apiospermum. Overall agreement between Etest and microdilution MICs was 84% for
Aspergillus spp. and 100% for the less common opportunistic molds, with the exception of
Penicillium spp. (67%). Where a discrepancy was observed between Etest and the reference method, the Etest tended to give lower values. The Etest method using RPMI agar appears to be a useful method for determining posaconazole susceptibilities of filamentous fungi.
Details
- Title: Subtitle
- In vitro susceptibility testing of filamentous fungi: comparison of Etest and reference M38-A microdilution methods for determining posaconazole MICs
- Creators
- M.A Pfaller - Department of Pathology, University of Iowa College of Medicine, and College of Public Health,Iowa City, Iowa 52242, USAS.A Messer - Department of Pathology, University of Iowa College of Medicine, and College of Public Health,Iowa City, Iowa 52242, USAL Boyken - Department of Pathology, University of Iowa College of Medicine, and College of Public Health,Iowa City, Iowa 52242, USAR.J Hollis - Department of Pathology, University of Iowa College of Medicine, and College of Public Health,Iowa City, Iowa 52242, USAD.J Diekema - Department of Pathology, University of Iowa College of Medicine, and College of Public Health,Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.45(4), pp.241-244
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0732-8893(02)00544-8
- PMID
- 12729993
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 2003
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9983986261502771
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