Journal article
In vivo DNA-protein cross-linking by cis- and trans-diamminedichloroplatinum(II)
Cancer research (Chicago, Ill.), Vol.47(1), pp.201-205
1987
PMID: 2431763
Abstract
When Novikoff hepatoma-bearing rats were given injections of a therapeutic dose of cis-diamminedichloroplatinum(II) (cis-DDP) (7 mg/kg), DNA-protein cross-links could be detected by using antisera to dehistonized chromatin, nuclear matrix, or Novikoff hepatoma cytoskeletal preparation. The extent of cross-linking increased in time up to 24 h after the injection, after which time the DNA-protein cross-links were gradually repaired, with no cross-links detectable at 72 h. trans-DDP in equitoxic (40 mg/kg) dose was very efficient in forming DNA-protein cross-links. Although formed more rapidly, these trans-DDP-mediated cross-links were repaired faster, within 48 h after the injection. The repair of cross-links at equimolar trans-DDP dose (7 mg/kg) was even more rapid. The principal proteins cross-linked to the DNA by both cis- or trans-DDP were Novikoff hepatoma cytokeratins (Mr 39,000, 49,000, 56,000, and an additional protein band reacting with the antiserum to Novikoff hepatoma cytoskeletal preparation at Mr approximately 68,000).
Details
- Title: Subtitle
- In vivo DNA-protein cross-linking by cis- and trans-diamminedichloroplatinum(II)
- Creators
- R OLINSKI - Vanderbilt univ. cancer center, A.B. Hancock Jr. memorial lab., Nashville TN37232, United StatesA WEDRYCHOWSKI - Vanderbilt univ. cancer center, A.B. Hancock Jr. memorial lab., Nashville TN37232, United StatesW. N SCHMIDT - Vanderbilt univ. cancer center, A.B. Hancock Jr. memorial lab., Nashville TN37232, United StatesR. C BRIGGS - Vanderbilt univ. cancer center, A.B. Hancock Jr. memorial lab., Nashville TN37232, United StatesL. S HNILICA - Vanderbilt univ. cancer center, A.B. Hancock Jr. memorial lab., Nashville TN37232, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.47(1), pp.201-205
- Publisher
- American Association for Cancer Research
- PMID
- 2431763
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1987
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094759702771
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